pS331/pS333-CMKLR2 (phospho-Chemerin Receptor 2 Antibody)

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Agonist-induced Serine331/Serine333 phosphorylation of Chemerin Receptor 2

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Order number: 7TM0082A
Content: 100 µl
Host: Rabbit

Serine331/Serine333 (S331/S333) is a major phosphorylation site of the Chemerin receptor 2... more

Serine331/Serine333 (S331/S333) is a major phosphorylation site of the Chemerin receptor 2 (CMKLR2). The pS331/pS333-CMKLR2 antibody detects phosphorylation in response to high-efficacy agonists. S331/S333 phosphorylation is a key regulator of CMKLR2 desensitization, β-arrestin recruitment and internalization.

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Alternative Names CMKLR2, Chemerin 2 IUPHAR Target ID 82... more

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Alternative Names	CMKLR2, Chemerin₂
IUPHAR Target ID	82
UniProt ID	P46091
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human CMKLR2 around the phosphorylation site Ser331/Ser333.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine331/Serine333 phosphorylation of Chemerin Receptor 2. Upper... more

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Figure 1. Agonist-induced Serine331/Serine333 phosphorylation of Chemerin Receptor 2. Upper panel, HEK293 cells stably expressing the Chemerin Receptor 2 (CMKLR2) were either not exposed or exposed to 100 nM Chemerin for 30 minutes. Cells were lysed and immunoblotted with the anti-pS331/pS333-CMKLR2 antibody (7TM0082A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CMKLR2 antibody to confirm equal loading of the gel.