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pS346/pS347-CXCR4 (phospho-CXC Chemokine Receptor 4 Antibody)

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  • 7TM0071B
  • 100 µl
  • Rabbit
Serine346/serine347 (S346/S347) is a major phosphorylation site of the CXCR4 receptor. The... more

Serine346/serine347 (S346/S347) is a major phosphorylation site of the CXCR4 receptor. The pS346/pS347-CXCR4 antibody detects phosphorylation in response to agonists and after PKC activation. S346/S347 phosphorylation is a key regulator of CXCR4 desensitization, β-arrestin recruitment and internalization.

  Alternative Names CXCR4, CXC Chemokine Receptor 4 IUPHAR Target ID... more

 

Alternative Names CXCR4, CXC Chemokine Receptor 4
IUPHAR Target ID  71
UniProt ID  P61073  (human) P70658 (mouse)
Western Blot (WB)  1:1000
Species Reactivity   Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human CXCR4 around the phosphorylation site of Ser346/Ser347
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine346/Serine347 phosphorylation of the CXC Chemokine Receptor 4.... more

Figure 1. Agonist-induced Serine346/Serine347 phosphorylation of the CXC Chemokine Receptor 4. Upper panel, HEK293 cells stably expressing the HA-tagged CXC Chemokine receptor 4 were either not exposed or exposed to 100 nM CXCL12 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS346/pS347-CXCR4 antibody (7TM0071B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Serine346/Serine347 CXC Chemokine Receptor 4 phosphorylation. Upper panel, HEK293 cells stably expressing the HA-tagged CXC Chemokine receptor 4 were either not exposed or exposed to increasing concentrations of CXCL12 ranging from 1 nM to 100 nM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS346/pS347-CXCR4 antibody (7TM0071B) at a dilution of 1:1000. Middle panel, blot was stripped and reprobed with the phosphorylation-sensitive anti-CXCR4 antibody (7TM0071N-WB) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000 to confirm equal loading of the gel.

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