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C5a1 (non-phospho), Complement C5a Receptor 1 Antibody

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  • 7TM0032N
  • 100 µl
  • Rabbit
The non-phospho-C5a1 receptor antibody is directed against the distal end of the... more

The non-phospho-C5a1 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human C5a1. It can be used to detect total C5a1 receptors in Western blots independent of phosphorylation. The non-phospho-C5a1 antibody can also be used to isolate and enrich C5a1 receptors from cell and tissue lysates. It also detects C5a1 in cultured cells and tissue sections by immunohistochemistry.

  Alternative Names C5a1, C5AR1, Complement C5a Receptor 1, C5a Anaphylatoxin... more

 

Alternative Names C5a1, C5AR1, Complement C5a Receptor 1, C5a Anaphylatoxin Chemotactic Receptor 1
IUPHAR Target ID  32
UniProt ID  P21730
Western Blot (WB)  1:1000
Immunocytochemistry (ICC) 1:200
Immunohistochemistry (IHC)  1:100
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide presents carboxyl-terminal tail of human C5a1
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Validation of the Complement C5a Receptor 1 in transfected HEK293 cells . Native... more

Figure 1. Validation of the Complement C5a Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the complement C5a receptor 1 (C5a1) were lysed and immunoblotted with the phosphorylation-independent anti-C5a1 antibody (7TM0032N-WB) at a dilution of 1:1000.

Figure 2. Immunocytochemical identification of Complement C5a Receptor 1 in HEK293 cells. HEK293 cells stably expressing the complement C5a receptor 1 (C5a1) were either not exposed or exposed to 1 μM C5a (complement fragment 5a) for 30 min and immunocytochemically stained with anti-C5a1 (non-phospho-Complement C5a Receptor 1) antibody (7TM0032N-WB) at a dilution of 1:200. Note, C5a1 receptors were confined to the plasma membrane in untreated cells (0 min). C5a1 receptors were seen in perinuclear clusters of vesicles after 30 min C5a1 exposure.

Figure 3. Immunohistochemical identification of Complement C5a receptor 1 in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-C5a1 (non-phospho-Complement C5a Receptor 1) antibody (7TM0032N-WB) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, C5a1 receptors were detected at the plasma membrane of a distinct population of cells in human spleen.

Figure 4. Immunohistochemical identification of Complement C5a receptor 1 in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-C5a1 (non-phospho-Complement C5a Receptor 1) antibody (7TM0032N-WB) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, C5a1 receptors were detected at the plasma membrane of a distinct population of cells in human spleen.

Figure 5. Immunohistochemical identification of Complement C5a Receptor 1 in human lung. Sections were dewaxed, microwaved in citric acid, and incubated with anti-C5a1 (non-phospho-Complement C5a Receptor 1) antibody (7TM00032N-WB) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, C5a1 receptors were uniformly detected at the plasma membrane of lung macrophages.

Nürge B, Schulz AL, Kaemmerer D, Sänger J, Evert K, Schulz S, Lupp A. Immunohistochemical... more

Nürge B, Schulz AL, Kaemmerer D, Sänger J, Evert K, Schulz S, Lupp A. Immunohistochemical identification of complement peptide C5a receptor 1 (C5aR1) in non-neoplastic and neoplastic human tissues. PLoS One. 2021 Feb 19;16(2):e0246939. doi: 10.1371/journal.pone.0246939. PMID: 33606748; PMCID: PMC7894821.

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