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- Order number: 7TM0033A
- Content: 100 µl
- Host: Rabbit
Serine326/Threonine327 (S326/T327) is major phosphorylation site of the Complement Peptide Receptor 2 (C5a2). The pS326/pT327-C5a2 antibody detects phosphorylation in response to agonists. S326/S327 phosphorylation is likely to be involved in efficient ligand sequestration by C5a2.
Alternative Names | C5a2, C5AR2, Complement C5a Receptor 2, C5a Anaphylatoxin Chemotactic Receptor 2 |
IUPHAR Target ID | 33 |
UniProt ID | Q9P296 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human C5a2 around the phosphorylation site of Ser326/Thr327 |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine326/Threonine327 phosphorylation of the Complement C5a Receptor 2. Upper panel, HEK293 cells stably expressing the Complement C5a Receptor 2 (C5a2) were either not exposed or exposed to 1 µM specific Complement C5a Receptor agonist CO28 or 100 nM C5a or 100 nM des-Arg C5a for 30 minutes. Cells were lysed and immunoblotted with the anti-pS326/pT327-C5a1 antibody (7TM0033A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-C5a2 antibody (7TM0033N) at a dilution of 1:1000 to confirm equal loading of the gel.
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