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pS326/pT327-C5a2 (phospho-Complement C5a Receptor 2 Antibody)

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Agonist-induced Serine326/Threonine327 phosphorylation of the Complement C5a Receptor 2.
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  • 7TM0033A
  • 100 µl
  • Rabbit
Serine326/Threonine327 (S326/T327) is major phosphorylation site of the Complement Peptide... more

Serine326/Threonine327 (S326/T327) is major phosphorylation site of the Complement Peptide Receptor 2 (C5a2). The pS326/pT327-C5a2 antibody detects phosphorylation in response to agonists. S326/S327 phosphorylation is likely to be involved in efficient ligand sequestration by C5a2.

  Alternative Names C5a2, C5AR2, Complement C5a Receptor 2, C5a Anaphylatoxin... more

 

Alternative Names C5a2, C5AR2, Complement C5a Receptor 2, C5a Anaphylatoxin Chemotactic Receptor 2
IUPHAR Target ID  33
UniProt ID  Q9P296
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human C5a2 around the phosphorylation site of Ser326/Thr327
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine326/Threonine327 phosphorylation of the Complement C5a Receptor... more

Figure 1. Agonist-induced Serine326/Threonine327 phosphorylation of the Complement C5a Receptor 2. Upper panel, HEK293 cells stably expressing the Complement C5a Receptor 2 (C5a2) were either not exposed or exposed to 1 µM specific Complement C5a Receptor agonist CO28 or 100 nM C5a or 100 nM des-Arg C5a for 30 minutes. Cells were lysed and immunoblotted with the anti-pS326/pT327-C5a1 antibody (7TM0033A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-C5a2 antibody (7TM0033N) at a dilution of 1:1000 to confirm equal loading of the gel.

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