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- Order number: 7TM0033MN-GP
- Content: 100 µl
- Host: Guinea Pig
The non-phospho-C5a2 receptor antibody is directed against the distal part of the carboxyl-terminal tail of mouse C5a2. It can be used to detect total C5a2 receptors in Western blots independent of phosphorylation. The non-phospho-C5a2 antibody can also be used to isolate and enrich C5a2 receptors from cell and tissue lysates.
| Alternative Names | C5a2, C5AR2, Complement C5a Receptor 2, C5a Anaphylatoxin Chemotactic Receptor 2 |
| IUPHAR Target ID | 33 |
| UniProt ID | Q8BW93 |
| Western Blot (WB) | 1:1000 |
| Species Reactivity | Mouse |
| Host / Isotype | Guinea Pig / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide presents carboxyl-terminal tail of mouse C5a2. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the mouse Complement C5a Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the mouse Complement C5a Receptor 2 (C5a2) were lysed and immunoblotted with the phosphorylation-independent anti-C5a2 antibody (7TM0033MN-GP) at a dilution of 1:1000.
Nürge B, Schulz AL, Kaemmerer D, Sänger J, Evert K, Schulz S, Lupp A. Immunohistochemical identification of complement peptide C5a receptor 1 (C5aR1) in non-neoplastic and neoplastic human tissues. PLoS One. 2021 Feb 19;16(2):e0246939. doi: 10.1371/journal.pone.0246939. PMID: 33606748; PMCID: PMC7894821.



