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- Order number: 7TM0212A
- Content: 100 µl
- Host: Rabbit
Threonine428/Serine429 (T428/S429) is a major phosphorylation site of the CRF1 receptor. The pT428/pS429-CRF1 antibody detects phosphorylation in response to high-efficacy agonists. T428/S429 phosphorylation is a key regulator of CRF1 desensitization, β-arrestin recruitment and internalization.
Alternative Names | CRFR1, CRH-R1, CRF-1R alpha, Corticotropin-Releasing Factor Receptor 1 |
IUPHAR Target ID | 212 |
UniProt ID | P34998 |
Western Blot (WB) | 1:1000 |
Immunocytochemistry (ICC) | - |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human CRF1 around the phosphorylation site Thr428/Ser429. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Threonine428/Serine429 phosphorylation of CRF1 Receptor. Upper panel, HEK293 cells stably expressing the CRF1-Receptor (CRF1) were either not exposed or exposed to 1 µM of specific CRF-Receptor agonist CRH for 30 minutes. Cells were lysed and immunoblotted with the anti-pT428/pS429-CRF1 antibody (7TM0212A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-CRF1 antibody to confirm equal loading of the gel.