pS434/pS437-CRF1 (phospho-Corticotropin-Releasing Factor Receptor 1 Antibody)

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Agonist-induced Serine434/Serine437 phosphorylation of CRF1 Receptor

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Order number: 7TM0212C
Content: 100 µl
Host: Rabbit

Serine434/Serine441 (S437/S441) is a major phosphorylation site of the CRF1 receptor.... more

Serine434/Serine441 (S437/S441) is a major phosphorylation site of the CRF1 receptor. The pS434/pS437-CRF1 antibody detects phosphorylation in response to high-efficacy agonists. S434/S437 phosphorylation is a key regulator of CRF1 desensitization, β-arrestin recruitment and internalization. S434/S437 also undergoes constitutive phosphorylation.

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Alternative Names CRFR1, CRH-R1, CRF-1R alpha, Corticotropin-Releasing... more

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Alternative Names	CRFR1, CRH-R1, CRF-1R alpha, Corticotropin-Releasing Factor Receptor 1
IUPHAR Target ID	212
UniProt ID	P34998
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human CRF1 around the phosphorylation site Ser434/Ser437.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced and Agonist-independent Serine434/Serine437 phosphorylation of CRF1... more

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Figure 1. Agonist-induced and Agonist-independent Serine434/Serine437 phosphorylation of CRF1 Receptor. Upper panel, HEK293 cells stably expressing the CRF1-Receptor (CRF1) were either not exposed or exposed to 1 µM of specific CRF-Receptor agonist CRH for 30 minutes. Cells were lysed and immunoblotted with the anti-pS434/pS437-CRF1 antibody (7TM0212C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-CRF1 antibody to confirm equal loading of the gel.

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