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- Order number: 7TM0248A
- Content: 100 µl
- Host: Rabbit
Threonine442/Serine443 (T442/S443) is major phosphorylation site of human GIP receptor. The pT442/pS443-GIPR antibody detects phosphorylation in response to agonists. T442/S443 phosphorylation is likely to be involved in efficient ligand sequestration by the Gastric Inhibitory Polypeptide Receptor.
Alternative Names | Gippr |
IUPHAR Target ID | 248 |
UniProt ID | P48546 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human GIPR around the phosphorylation site of Thr442/Ser443. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Threonine442/Serine443 phosphorylation of GIPR. Upper panel, HEK293 cells stably expressing the GIP-Receptor (GIPR) were either not exposed or exposed to 1 µM of GIP receptor agonist GIP for 30 minutes. Cells were lysed and immunoblotted with the anti-pT442/pS443-GIPR antibody (7TM0248A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-GIPR antibody to confirm equal loading of the gel.