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pT442/pS443-GIPR (phospho-Gastric Inhibitory Polypeptide Receptor Antibody)

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pT442/pS443-GIPR (phospho-Gastric Inhibitory Polypeptide Receptor Antibody)
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  • 7TM0248A
  • 100 µl
  • Rabbit
Threonine442/Serine443 (T442/S443) is major phosphorylation site of human GIP receptor. The... more

Threonine442/Serine443 (T442/S443) is major phosphorylation site of human GIP receptor. The pT442/pS443-GIPR antibody detects phosphorylation in response to agonists. T442/S443 phosphorylation is likely to be involved in efficient ligand sequestration by the Gastric Inhibitory Polypeptide Receptor.

  Alternative Names Gippr IUPHAR Target ID   248... more

 

Alternative Names Gippr
IUPHAR Target ID  248
UniProt ID  P48546
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human GIPR around the phosphorylation site of Thr442/Ser443.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threonine442/Serine443 phosphorylation of GIPR.   Upper panel,... more

Figure 1. Agonist-induced Threonine442/Serine443 phosphorylation of GIPR. Upper panel, HEK293 cells stably expressing the GIP-Receptor (GIPR) were either not exposed or exposed to 1 µM of  GIP receptor agonist GIP for 30 minutes. Cells were lysed and immunoblotted with the anti-pT442/pS443-GIPR antibody (7TM0248A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-GIPR antibody to confirm equal loading of the gel.

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