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- Order number: 7TM0250N-IC
- Content: 100 µl
- Host: Rabbit
The non-phospho GLP2 receptor antibody is directed against the distal end of the carboxyl-terminal tail human GLP2 receptor. It can be used to detect total GLP2 receptors in Western blots independent of phosphorylation. It can also be used to isolate and enrich GLP2 receptors from tissue lysates. It also detects GLP2 in cultured cells and tissue sections by immunohistochemistry.
| Alternative Names | GLP-2R |
| IUPHAR Target ID | 250 |
| UniProt ID | O958383 |
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide corresponding to c-terminal end of human GLP2. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Immunohistochemical identification of Glucagon-Like Peptide 2 Receptor in enteric ganglion. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GLP2 (non-phospho-Glucagon-Like Peptide 2 Receptor) antibody (7TM0250N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.
Figure 2. Validation of the Glucagon-Like Peptide 2 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Glucagon-Like Peptide 2 Receptor (GLP2) were lysed and immunoblotted with the anti-GLP2 antibody (7TM0250N-IC) at a dilution of 1:1000.
Figure 3. Immunohistochemical varification of Glucagon-Like Peptide 2 Receptor antibody in duodenum. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-GLP2 (non-phospho-Glucagon-Like Peptide 2 Receptor) antibody (7TM0250N-IC). Sections were then incubated with anti-S1P1 (non-phospho-Glucagon-Like Peptide 2 Receptor) antibody (7TM0250N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation GLP2 receptors were uniformly detected at the plasma membrane of nearly all cells.
