pS298/pS300-M4 (IHC-grade phospho-M4 Muscarinic Acetylcholine Receptor Antibody)

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Preview: Immunohistochemical identification of Serine298/Serine300 phosphorylation of M4 Receptor in striatum

Preview: Agonist-induced Serine298/Serine300 phosphorylation of the M4 Muscarinic Acetylcholine Receptor

Immunohistochemical identification of Serine298/Serine300 phosphorylation of M4 Receptor in striatum

Agonist-induced Serine298/Serine300 phosphorylation of the M4 Muscarinic Acetylcholine Receptor

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Order number: 7TM0016D-IC
Content: 300 µl
Host: Rabbit

Serine298/Serine300 is a major phosphorylation site of the M4 Muscarinic Acetylcholine Receptor.... more

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Serine298/Serine300 is a major phosphorylation site of the M4 Muscarinic Acetylcholine Receptor. The pS298/pS300-M4 antibody detects phosphorylation in response to high-efficacy agonists but not after PKC activation. S298/S300 phosphorylation is a key regulator of M4 desensitization, β-arrestin recruitment and internalization. pS298/pS300-antibody can detect phosphorylated M4 in mouse brain in vivo.

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Alternative Names HM3, Chrm 4, Cholinergic Receptor muscarinic 4... more

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Alternative Names	HM3, Chrm 4, Cholinergic Receptor muscarinic 4
IUPHAR Target ID	16
UniProt ID	P08173
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human M4 around the phosphorylation site of Ser298/Ser300.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of... more

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Figure 1. Immunohistochemical identification of Serine298/Serine300 phosphorylation of M4 Receptor in striatum. Sections were dewaxed, microwaved in citric acid, and incubated with anti-pS298/pS300-M4 (M4 Receptor) antibody (7TM0016D-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Agonist-induced Serine298/Serine300 phosphorylation of the M4 Muscarinic Acetylcholine Receptor. Upper panel, HEK293 cells stably expressing the M4 Muscarinic Acetylcholine Receptor (M4) and high level of GRK2 were either not exposed or exposed to 100 µM Carbachol for 30 minutes. Cells were lysed and immunoblotted according to 7TM Western Blot Protocol with the anti-pS298/pS300-M4 antibody (7TM0016D-IC) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-M4 antibody (7TM0016N) to confirm equal loading of the gel.