pT271-M2 (phospho-M2 Muscarinic Acetylcholine Receptor Antibody)

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Agonist-induced Threonine271 phosphorylation of the M2 Muscarinic Acetycholine Receptor

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Order number: 7TM0014A
Content: 100 µl
Host: Rabbit

Threonine271 is a major phosphorylation site of the M2 Muscarinic Acetylcholine Receptor. The... more

Threonine271 is a major phosphorylation site of the M2 Muscarinic Acetylcholine Receptor. The pT271-M2 antibody detects phosphorylation in response to high-efficacy agonists but not after PKC activation. T271 phosphorylation is a key regulator of M2 desensitization, β-arrestin recruitment and internalization.

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Alternative Names AChR M2, Chrm 2, Cholinergic Receptor muscarinic 2... more

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Alternative Names	AChR M2, Chrm 2, Cholinergic Receptor muscarinic 2
IUPHAR Target ID	14
UniProt ID	P08172
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human M2 around the phosphorylation site of Thr271.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine271 phosphorylation of the M2 Muscarinic Acetycholine... more

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Figure 1. Agonist-induced Threonine271 phosphorylation of the M2 Muscarinic Acetycholine Receptor. Upper panel, HEK293 cells stably expressing the M2 Muscarinic Acetycholine Receptor (M2) were either not exposed or exposed to 100 nM Carbachol for 30 minutes. Cells were lysed and immunoblotted with the anti-pT271-M2 antibody (7TM0014A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-M2 antibody to confirm equal loading of the gel.

Be the first to decipher the M2 phosphorylation barcode and let us know. more

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Be the first to decipher the M2 phosphorylation barcode and let us know.

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