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- Order number: 7TM0013A
- Content: 100 µl
- Host: Rabbit
Serine298 is a major phosphorylation site of the M1 muscarinic acetylcholine receptor. The pS298-M1 antibody detects phosphorylation in response to high-efficacy agonists.
Alternative Names | M1R, Cholinergic Receptor, Cholinergic Receptor muscarinic 1 |
IUPHAR Target ID | 13 |
UniProt ID | P11229 |
Western Blot (WB) | 1:1000 |
Immunocytochemistry (ICC) | - |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human M1 around the phosphorylation site of Ser298. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine298 phosphorylation of the M1 Muscarinic Acetylcholine Receptor. Upper panel, HEK293 cells stably expressing the M1 Muscarinic Acetycholine Receptor (M1) were either not exposed or exposed to 100 µM acetylcholine for 30 minutes. Cells were lysed and immunoblotted with the anti-pS298-M1 antibody (7TM0013A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-M1 antibody to confirm equal loading of the gel.
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