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pS298-M1 (phospho-M1 Muscarinic Acetylcholine Receptor Antibody)

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Agonist-induced Serine298 phosphorylation of the M1 Muscarinic Acetylcholine Receptor
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  • 7TM0013A
  • 100 µl
  • Rabbit
Serine298 is a major phosphorylation site of the M1 muscarinic acetylcholine receptor.... more

Serine298 is a major phosphorylation site of the M1 muscarinic acetylcholine receptor. The pS298-M1 antibody detects phosphorylation in response to high-efficacy agonists.

  Alternative Names M1R, Cholinergic Receptor, Cholinergic Receptor... more

 

Alternative Names M1R, Cholinergic Receptor, Cholinergic Receptor muscarinic 1
IUPHAR Target ID  13
UniProt ID  P11229
Western Blot (WB)  1:1000
Immunocytochemistry (ICC)  -
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human M1 around the phosphorylation site of Ser298.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine298 phosphorylation of the M1 Muscarinic Acetylcholine... more

Figure 1. Agonist-induced Serine298 phosphorylation of the M1 Muscarinic Acetylcholine Receptor. Upper panel, HEK293 cells stably expressing the M1 Muscarinic Acetycholine Receptor (M1) were either not exposed or exposed to 100 µM acetylcholine for 30 minutes. Cells were lysed and immunoblotted with the anti-pS298-M1 antibody (7TM0013A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-M1 antibody to confirm equal loading of the gel.

Be the first to decipher the M1 phosphorylation barcode and let us know. more

Be the first to decipher the M1 phosphorylation barcode and let us know.

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