pS332/pS333/pS334-M3 (phospho-M3 Muscarinic Acetylcholine Receptor Antibody)

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Agonist-induced Serine332/Serine333/Serine334 phosphorylation of the M3 Muscarinic Acetycholine Receptor.

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Order number: 7TM0015A
Content: 100 µl
Host: Rabbit

Serine332/Serine333/Serine334 is a major phosphorylation site of the M3 Muscarinic Acetylcholine... more

Serine332/Serine333/Serine334 is a major phosphorylation site of the M3 Muscarinic Acetylcholine Receptor. The pS332/pS333/pS334-M3 antibody detects phosphorylation in response to high-efficacy agonists but not after PKC activation. S332/S333/S334 phosphorylation is a key regulator of M3 desensitization, β-arrestin recruitment and internalization.

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Alternative Names HM4, Chrm 3, Cholinergic Receptor muscarinic 3... more

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Alternative Names	HM4, Chrm 3, Cholinergic Receptor muscarinic 3
IUPHAR Target ID	15
UniProt ID	P20309
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human M3 around the phosphorylation site of Ser332/Ser333/Ser334.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine332/Serine333/Serine334 phosphorylation of the M3 Muscarinic... more

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Figure 1. Agonist-induced Serine332/Serine333/Serine334 phosphorylation of the M3 Muscarinic Acetycholine Receptor. Upper panel, HEK293 cells stably expressing the M3 Muscarinic Acetycholine Receptor (M3) were either not exposed or exposed to 100 nM Acetylcholine for 30 minutes. Cells were lysed and immunoblotted with the anti-pS332/pS333/pS334-M3 antibody (7TM0015A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-M3 antibody to confirm equal loading of the gel.

Be the first to decipher the M3 phosphorylation barcode and let us know. more

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Be the first to decipher the M3 phosphorylation barcode and let us know.

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