pS354/pT356-NPY2 (phospho-Neuropeptide Y Receptor 2 Antibody)

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Preview: Agonist-induced Serine354/Threonine356 phosphorylation of the Neuropeptide Y receptor 2

Preview: Analysis of dose-dependent NPY2 receptor phosphorylation using a panel of phosphosite-specific antibodies

Agonist-induced Serine354/Threonine356 phosphorylation of the Neuropeptide Y receptor 2

Analysis of dose-dependent NPY2 receptor phosphorylation using a panel of phosphosite-specific antibodies

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Order number: 7TM0306B
Content: 100 µl
Host: Rabbit

Serine354Threonine356 (S354/T356) is a major phosphorylation site of the Neuropeptide Y Receptor... more

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Serine354Threonine356 (S354/T356) is a major phosphorylation site of the Neuropeptide Y Receptor 2. The pS354/pT356-NPY2 antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. S354/T356 phosphorylation is primarily mediated by GRKs and is a key regulator of NPY2 desensitization, β-arrestin recruitment and internalization.

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Alternative Names Y2, NPY 2 receptor, neuropeptide Y receptor type 2... more

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Alternative Names	Y2, NPY 2 receptor, neuropeptide Y receptor type 2
IUPHAR Target ID	306
UniProt ID	P49146
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human NPY2 around the phosphorylation site of Ser354/Thr356.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine354/Threonine356 phosphorylation of the Neuropeptide Y receptor... more

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Figure 1. Agonist-induced Serine354/Threonine356 phosphorylation of the Neuropeptide Y receptor 2. Upper panel, HEK293 cells stably expressing the NPY2-Receptor (NPY2) were either not exposed or exposed to 1 µM of NPY2 receptor agonist Neuropeptide Y for 30 minutes. Cells were lysed and immunoblotted with the anti-pS354/pT356-NPY2 antibody (7TM0306B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-NPY2 antibody to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent NPY2 receptor phosphorylation using a panel of phosphosite-specific antibodies. Upper four panels, HEK293 cells stably expressing the NPY2 receptor (NPY2) were either not exposed or exposed to increasing concentrations of Neuropeptide Y ranging from 100 pM to 1 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS351/pS354-NPY2 antibody (7TM0306A) or anti-pS354/pT356-NPY2 antibody (7TM0306B) or anti-pS369/pS374-NPY2 antibody (7TM0306C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-NPY2 antibody to confirm equal loading of the gel.

Be the first to decipher the NPY2 phosphorylation barcode and let us know. more

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Be the first to decipher the NPY2 phosphorylation barcode and let us know.

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