![Agonist-induced Serine409/Serine410 phosphorylation of the Neurotensin Receptor 1](https://7tmantibodies.com/media/image/01/72/6e/pS409-PS410-NTS1-F1small_600x600.png)
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- Order number: 7TM0309C
- Content: 100 µl
- Host: Rabbit
Serine409/Serine410 (S409/S410) is major phosphorylation site of the Neurotensin Receptor 1 (NTS1). The pS409/pS410-NTS1 antibody detects phosphorylation in response to agonists. S409/S410 phosphorylation is likely to be involved in efficient ligand sequestration by NTS1.
Alternative Names | NTS1, NTR1, Neurotensin Receptor Type 1, Neurotensin Receptor 1 |
IUPHAR Target ID | 309 |
UniProt ID | P30989 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human NTS1 around the phosphorylation site of Ser409/Ser410. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine409/Serine410 phosphorylation of the Neurotensin Receptor 1. Upper panel, HEK293 cells stably expressing the Neurotensin receptor 1 (NTS1) were either not exposed or exposed to 100 nM Neurotensin for 30 minutes. Cells were lysed and immunoblotted with the anti-pS409/pS410-NTS1 antibody (7TM0309C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-NTS1 antibody (7TM0309N) to confirm equal loading of the gel.
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