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- Order number: 7TM0088N
- Content: 100 µl
- Host: Rabbit
The non-phospho-GPR17 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human GPR17. It can be used to detect total GPR17 receptors in Western blots independent of phosphorylation. The non-phospho-GPR17 antibody can also be used to detect GPR17 in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.
| Alternative Names | P2Y-like receptor, uracil nucleotide/cysteinyl leukotriene receptor |
| IUPHAR Target ID | 88 |
| UniProt ID | Q13304 |
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide presents the carboxyl-terminal tail of human GPR17. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the G Protein-coupled Receptor 17 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the G Protein-coupled Receptor 17 (GPR17) were lysed and immunoblotted with the phosphorylation-independent anti-GPR17 antibody (7TM0088N) at a dilution of 1:1000.
Figure 2. Immunohistochemical identification of G Protein-coupled Receptor 17 in oligodendrocytes. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR17 (G Protein-coupled Receptor 17) antibody (7TM0088N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR17 receptors were detected at the plasma membrane of cells.
