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- Order number: 7TM0637A
- Content: 100 µl
- Host: Rabbit
Serine358/Serine362 (S358/S362) is a major phosphorylation site of the human GPR176 receptor. The pS358/pS362-GPR176 antibody detects GRK-mediated constitutive phosphorylation as well as PKC-mediated phosphorylation of GPR176 when expressed in HEK293 cells.
| Alternative Names | HB954, G-protein coupled receptor AGR9, RBU-15 |
| IUPHAR Target ID | 637 |
| UniProt ID | Q14439 |
| Western Blot (WB) | 1:1000 |
| Immunocytochemistry (ICC) | - |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic phosphopeptide derived from human GPR176 around the phosphorylation site Ser358/Ser362. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine358/Seine362 phosphorylation of the G-protein coupled Receptor 176. Upper panel, HEK293 cells stably expressing the G-protein coupled receptor 176 (GPR176) are marked as wildtype (WT). HEK293 cells stably expressing the G-protein coupled receptor 176 (GPR176) and have full knock-out of G protein-coupled receptor kinases (GRK) 2,3,4 and 5 are marked as ΔGRK2/3/4/5. Knock-out cells were treated or not treated with 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) or 1 µM Forskolin. Cells were lysed and immunoblotted with the anti-pS358/pS362-GPR176 antibody (7TM0637A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-GPR176 antibody to confirm equal loading of the gel.