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- Order number: 7TM0097N
- Content: 100 µl
- Host: Rabbit
The non-phospho-GPR27 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human GPR27. It can be used to detect total GPR27 receptors in Western blots independent of phosphorylation. The GPR27 antibody can also be used to isolate and enrich GPR27 receptors from tissue lysates.
| Alternative Names | SREB1 |
| IUPHAR Target ID | 97 |
| Uniprot ID | Q9NS26 |
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide corresponding to residues distal end of carboxy-terminal tail of human GPR27. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the GPR27 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR27 Receptor (GPR27) were lysed and immunoblotted with the phosphorylation-independent anti-GPR27 antibody (7TM0097N) at a dilution of 1:1000.
Figure 2. Immunohistochemical identification of GPR27 Receptor in small intestine. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR27 (GPR27 Receptor) antibody (7TM0097N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.