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pS300/pS303-hGPR35 (phospho-human GPR35 Antibody)

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  • 7TM0102A
  • 100 µl
  • Rabbit
Serine300/Serine303 (S300/S303) is a major phosphorylation site of the human GPR35 receptor.... more

Serine300/Serine303 (S300/S303) is a major phosphorylation site of the human GPR35 receptor. The pS300/pS303-hGPR35 antibody detects phosphorylation in response to high-efficacy agonists. S300/S303 phosphorylation is a key regulator of human GPR35 desensitization, β-arrestin recruitment and internalization.

  Alternative Names GPR35, G-protein coupled Receptor 35 IUPHAR Target... more

 

Alternative Names GPR35, G-protein coupled Receptor 35
IUPHAR Target ID  102
UniProt ID  Q9HC97
Western Blot (WB)  1:1000
Immunocytochemistry (ICC) 1:200
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human GPR35 around the phosphorylation site Ser300/Ser303.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine300/Serine303 phosphorylation of GPR35 Receptor. Upper panel,... more

Figure 1. Agonist-induced Serine300/Serine303 phosphorylation of GPR35 Receptor. Upper panel, HEK293 cells transiently expressing the eYFP-tagged human GPR35-Receptor were either not exposed (vehicle) or exposed to 100 nM of specific GPR35-Receptor agonist zaprinast for 5 minutes. Cells were lysed and receptor constuct enriched with a GFP-trap before immunoblotting with the anti-pS300/pS303-hGPR35 antibody (7TM0102A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-GFP antibody to confirm equal loading of the gel.

Figure 2. Immunocytochemical Identification of Serine300/Serine303 phosphorylation of GPR35 Receptor. Flp-ln TREx 293 cells stably expressing hGPR35a-HA were either treated with vehicle or 100 μM zaprinast for 5 min and immunocytochemically stained with anti-pS300/pS303-hGPR35 antibody (7TM0102A) at a dilution of 1:200.

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