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pS300/pS303-hGPR35 (phospho-human GPR35 Antibody)

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  • 7TM0102A
  • 100 µl
  • Rabbit
Serine300/Serine303 (S300/S303) is a major phosphorylation site of the human GPR35 receptor.... more

Serine300/Serine303 (S300/S303) is a major phosphorylation site of the human GPR35 receptor. The pS300/pS303-hGPR35 antibody detects phosphorylation in response to high-efficacy agonists. S300/S303 phosphorylation is a key regulator of human GPR35 desensitization, β-arrestin recruitment and internalization.

  Alternative Names GPR35, G-protein coupled Receptor 35 IUPHAR Target... more

 

Alternative Names GPR35, G-protein coupled Receptor 35
IUPHAR Target ID  102
UniProt ID  Q9HC97
Western Blot (WB)  1:1000
Immunocytochemistry (ICC) 1:200
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human GPR35 around the phosphorylation site Ser300/Ser303.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine300/Serine303 phosphorylation of GPR35 Receptor. Upper panel,... more

Figure 1. Agonist-induced Serine300/Serine303 phosphorylation of GPR35 Receptor. Upper panel, HEK293 cells transiently expressing the eYFP-tagged human GPR35-Receptor were either not exposed (vehicle) or exposed to 100 nM of specific GPR35-Receptor agonist zaprinast for 5 minutes. Cells were lysed and receptor constuct enriched with a GFP-trap before immunoblotting with the anti-pS300/pS303-hGPR35 antibody (7TM0102A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-GFP antibody to confirm equal loading of the gel.

Figure 2. Immunocytochemical Identification of Serine300/Serine303 phosphorylation of GPR35 Receptor. Flp-ln TREx 293 cells stably expressing hGPR35a-HA were either treated with vehicle or 100 μM zaprinast for 5 min and immunocytochemically stained with anti-pS300/pS303-hGPR35 antibody (7TM0102A) at a dilution of 1:200.

Divorty N, Jenkins L, Ganguly A, Butcher AJ, Hudson BD, Schulz S, Tobin AB, Nicklin SA,... more

Divorty N, Jenkins L, Ganguly A, Butcher AJ, Hudson BD, Schulz S, Tobin AB, Nicklin SA, Milligan G. Agonist-induced phosphorylation of orthologues of the orphan receptor GPR35 functions as an activation sensor. J Biol Chem. 2022 Mar;298(3):101655. doi: 10.1016/j.jbc.2022.101655. Epub 2022 Jan 29. PMID: 35101446; PMCID: PMC8892012.

Ganguly A, Quon T, Jenkins L, Joseph B, Al-Awar R, Chevigne A, Tobin AB, Uehling DE, Hoffmann C, Drube J, Milligan G. G protein-receptor kinases 5/6 are the key regulators of G protein-coupled receptor 35-arrestin interactions. J Biol Chem. 2023 Oct;299(10):105218. doi: 10.1016/j.jbc.2023.105218. Epub 2023 Sep 1. PMID: 37660910; PMCID: PMC10520886.

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