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- Order number: 7TM0102A
- Content: 100 µl
- Host: Rabbit
Serine300/Serine303 (S300/S303) is a major phosphorylation site of the human GPR35 receptor. The pS300/pS303-hGPR35 antibody detects phosphorylation in response to high-efficacy agonists. S300/S303 phosphorylation is a key regulator of human GPR35 desensitization, β-arrestin recruitment and internalization.
| Alternative Names | GPR35, G-protein coupled Receptor 35 |
| IUPHAR Target ID | 102 |
| UniProt ID | Q9HC97 |
| Western Blot (WB) | 1:1000 |
| Immunocytochemistry (ICC) | 1:200 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic phosphopeptide derived from human GPR35 around the phosphorylation site Ser300/Ser303. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine300/Serine303 phosphorylation of GPR35 Receptor. Upper panel, HEK293 cells transiently expressing the eYFP-tagged human GPR35-Receptor were either not exposed (vehicle) or exposed to 100 nM of specific GPR35-Receptor agonist zaprinast for 5 minutes. Cells were lysed and receptor constuct enriched with a GFP-trap before immunoblotting with the anti-pS300/pS303-hGPR35 antibody (7TM0102A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-GFP antibody to confirm equal loading of the gel.
Figure 2. Immunocytochemical Identification of Serine300/Serine303 phosphorylation of GPR35 Receptor. Flp-ln TREx 293 cells stably expressing hGPR35a-HA were either treated with vehicle or 100 μM zaprinast for 5 min and immunocytochemically stained with anti-pS300/pS303-hGPR35 antibody (7TM0102A) at a dilution of 1:200.
