pS300/pS303-hGPR35 (phospho-human GPR35 Antibody)

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Preview: Agonist-induced Serine300/Serine303 phosphorylation of GPR35 Receptor.

Preview: Immunocytochemical Identification of Serine300/Serine303 phosphorylation of human GPR35 Receptor

Agonist-induced Serine300/Serine303 phosphorylation of GPR35 Receptor.

Immunocytochemical Identification of Serine300/Serine303 phosphorylation of human GPR35 Receptor

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Order number: 7TM0102A
Content: 100 µl
Host: Rabbit

Serine300/Serine303 (S300/S303) is a major phosphorylation site of the human GPR35 receptor.... more

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Serine300/Serine303 (S300/S303) is a major phosphorylation site of the human GPR35 receptor. The pS300/pS303-hGPR35 antibody detects phosphorylation in response to high-efficacy agonists. S300/S303 phosphorylation is a key regulator of human GPR35 desensitization, β-arrestin recruitment and internalization.

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Alternative Names GPR35, G-protein coupled Receptor 35 IUPHAR Target... more

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Alternative Names	GPR35, G-protein coupled Receptor 35
IUPHAR Target ID	120
UniProt ID	Q9HC97
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human GPR35 around the phosphorylation site Ser300/Ser303.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine300/Serine303 phosphorylation of GPR35 Receptor. Upper panel,... more

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Figure 1. Agonist-induced Serine300/Serine303 phosphorylation of GPR35 Receptor. Upper panel, HEK293 cells stably expressing the human GPR35-Receptor (NK1) were either not exposed or exposed to 1 µM of specific NK1-Receptor agonist Substance P for 30 minutes. Cells were lysed and immunoblotted with the anti-pT344/pS347-NK1 antibody (7TM0360A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-NK1 antibody (7TM0360N) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Immunocytochemical Identification of Serine300/Serine303 phosphorylation of human GPR35 Receptor. Flp-ln TREx 293 cells stably expressing human GPR35a-HA were either treated with vehicle or 100 μM zaprinast for 5 min and immunocytochemically stained with anti-pS300/pS303-mGPR35 antibody (7TM0102A) at a dilution of 1:200.

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