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pT347/pT349-GPR183 (phospho-GPR183 Antibody)

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Agonist-induced Threoine347/Serine349 phosphorylation of GPR183
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  • 7TM0081B
  • 100 µl
  • Rabbit
Threonine347/Threonine349 (T347/T349) is major phosphorylation site of human GPR183 receptor.... more

Threonine347/Threonine349 (T347/T349) is major phosphorylation site of human GPR183 receptor. The pT347/pT349-GPR183 antibody detects phosphorylation in response to agonists. T347/T349 phosphorylation is likely to be involved in efficient ligand sequestration by GPR183.

  Alternative Names EBI2, EBV-induced G-protein coupled receptor 2,... more

 

Alternative Names EBI2, EBV-induced G-protein coupled receptor 2, lymphocyte-specific G-protein coupled receptor
IUPHAR Target ID  81
UniProt ID  P32249
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human GPR183 around the phosphorylation site of Thr347/Thr349.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threoine347/Serine349 phosphorylation of GPR183.   Upper... more

Figure 1. Agonist-induced Threoine347/Serine349 phosphorylation of GPR183. Upper panel, HEK293 cells transiently expressing the human GPR183-Receptor were either not exposed (vehicle) or exposed to 100 nM of specific GPR183-Receptor agonist 7α,25-OHC for 5 minutes. Cells were lysed and immunoblotted with the anti-pT347/pT349-GPR183 antibody (7TM0081B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-GRP183 antibody to confirm equal loading of the gel.

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