![Agonist-induced Threoine347/Serine349 phosphorylation of GPR183](https://7tmantibodies.com/media/image/bc/a7/cd/pT347-pT349-GPR183-F1_600x600.png)
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- Order number: 7TM0081B
- Content: 100 µl
- Host: Rabbit
Threonine347/Threonine349 (T347/T349) is major phosphorylation site of human GPR183 receptor. The pT347/pT349-GPR183 antibody detects phosphorylation in response to agonists. T347/T349 phosphorylation is likely to be involved in efficient ligand sequestration by GPR183.
Alternative Names | EBI2, EBV-induced G-protein coupled receptor 2, lymphocyte-specific G-protein coupled receptor |
IUPHAR Target ID | 81 |
UniProt ID | P32249 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human GPR183 around the phosphorylation site of Thr347/Thr349. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Threoine347/Serine349 phosphorylation of GPR183. Upper panel, HEK293 cells transiently expressing the human GPR183-Receptor were either not exposed (vehicle) or exposed to 100 nM of specific GPR183-Receptor agonist 7α,25-OHC for 5 minutes. Cells were lysed and immunoblotted with the anti-pT347/pT349-GPR183 antibody (7TM0081B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-GRP183 antibody to confirm equal loading of the gel.