![Agonist-induced Threonine351 phosphorylation of the P2Y Receptor 4](https://7tmantibodies.com/media/image/de/b8/4a/pT351-P2Y4-F1_600x600.png)
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- Order number: 7TM0325C
- Content: 100 µl
- Host: Rabbit
Threonine351 (T351) is a major phosphorylation site of the P2Y4 receptor. The pT351-P2Y4 antibody detects phosphorylation in response to high- and low-efficacy agonists and after PKC activation. T351 phosphorylation is a key regulator of P2Y4 desensitization, β-arrestin recruitment and internalization.
Alternative Names | P2Y4, P2Y Purinoceptor 4, Pyrimidinergic Receptor P2Y4 |
IUPHAR Target ID | 325 |
UniProt ID | P51582 |
Western Blot (WB) | 1:1000 |
Immunocytochemistry (ICC) | - |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human P2Y4 around the phosphorylation site of Thr351. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Threonine351 phosphorylation of the P2Y Receptor 4. Upper panel, HEK293 cells stably expressing the P2Y Receptor 4 (P2Y4) were either not exposed or exposed to 1 µM ATP (Adenosintriphosphate) or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pT351-P2Y4 antibody (7TM00325C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-P2Y4 antibody to confirm equal loading of the gel.
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