pS364/pS366-EP4 (phospho-EP4 Prostanoid Receptor Antibody)

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Agonist-induce Serine364/Serine366 phosphorylation of the EP4 Prostanoid Receptor

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Order number: 7TM0343C
Content: 100 µl
Host: Rabbit

Serine364/Serine366 (S364/S366) is major phosphorylation site of the EP4 Prostanoid Receptor... more

Serine364/Serine366 (S364/S366) is major phosphorylation site of the EP4 Prostanoid Receptor (EP4). The pS364/pS366-EP4 antibody detects phosphorylation in response to agonists. S364/S366 phosphorylation is likely to be involved in efficient ligand sequestration by EP4.

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Alternative Names PGE Receptor EP4 subtype, Prostanoid EP4 Receptor... more

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Alternative Names	PGE Receptor EP4 subtype, Prostanoid EP4 Receptor
IUPHAR Target ID	343
UniProt ID	P35408
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human EP around the phosphorylation site of Ser364/Ser366.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induce Serine364/ Serine366 phosphorylation of the EP4 Prostanoid... more

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Figure 1. Agonist-induce Serine364/Serine366 phosphorylation of the EP4 Prostanoid Receptor. Upper panel, HEK293 cells stably expressing the EP4 Prostanoid receptor (EP4) were either not exposed or exposed to 10 µM Misoprostol for 30 minutes. Cells were lysed and immunoblotted with the anti-pS364/pS366-EP4 antibody (7TM0343C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-EP4 antibody to confirm equal loading of the gel.

Be the first to analyze the EP4 phosphorylation using our anti-pS364/pS366-EP4... more

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Be the first to analyze the EP4 phosphorylation using our anti-pS364/pS366-EP4 antibody and let us know.

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