pS383/pS384-PAR2 (phospho-Proteinase-Activated Receptor 2 Antibody)

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Agonist-induced Serine383/Serine384 phosphorylation of the Proteinase-Activated Receptor 2

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Order number: 7TM0348B
Content: 100 µl
Host: Rabbit

Serine383/Serine384 (S383/S384) is major phosphorylation site of the Proteinase-Activated... more

Serine383/Serine384 (S383/S384) is major phosphorylation site of the Proteinase-Activated Receptor 2 (PAR2). The pS383/pS384-PAR2 antibody detects phosphorylation in response to agonists. S383/S384 phosphorylation is likely to be involved in efficient ligand sequestration by PAR2.

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Alternative Names Coagulation Factor II Receptor-like 1, Protease-Activated... more

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Alternative Names	Coagulation Factor II Receptor-like 1, Protease-Activated Receptor 2
IUPHAR Target ID	348
UniProt ID	P55085
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human PAR2 around the phosphorylation site of Ser383/Ser384.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine383/Serine384 phosphorylation of the Proteinase-Activated... more

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Figure 1. Agonist-induced Serine383/Serine384 phosphorylation of the Proteinase-Activated Receptor 2. Upper panel, HEK293 cells stably expressing the Proteinase-activated receptor 2 (PAR2) were either not exposed or exposed to 100 nM AC264613 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS383/pS384-PAR2 antibody (7TM0348B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-PAR2 antibody to confirm equal loading of the gel.

Be the first to analyze the ACKR2 phosphorylation using our anti-pS348/pS350-ACKR2... more

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Be the first to analyze the ACKR2 phosphorylation using our anti-pS348/pS350-ACKR2 antibody and let us know.

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