pS366/pS369-PAR4 (phospho-Proteinase-Activated Receptor 4 Antibody)

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Agonist-induced Serine366/Serine369 phosphorylation of the Proteinase-Activated Receptor 4

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Order number: 7TM0350A
Content: 100 µl
Host: Rabbit

Serine366/Serine369 (S366/S369) is a major phosphorylation site of the Proteinase-Activated... more

Serine366/Serine369 (S366/S369) is a major phosphorylation site of the Proteinase-Activated Receptor 4 (PAR4). The pS366/pS369-PAR4 antibody detects phosphorylation in response to agonists. S366/S369 phosphorylation is likely to be involved in efficient ligand sequestration by PAR4.

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Alternative Names Coagulation Factor II Receptor-like 3, Protease-Activated... more

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Alternative Names	Coagulation Factor II Receptor-like 3, Protease-Activated Receptor 4, Thrombin receptor-like 3
IUPHAR Target ID	350
UniProt ID	Q96RI0
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human PAR4 around the phosphorylation site of Ser366/Ser369.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine366/Serine369 phosphorylation of the Proteinase-Activated... more

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Figure 1. Agonist-induced Serine366/Serine369 phosphorylation of the Proteinase-Activated Receptor 4. Upper panel, HEK293 cells stably expressing the Proteinase-activated receptor 4 (PAR4) were either not exposed or exposed to 100 nM AYPGKF for 30 minutes. Cells were lysed and immunoblotted with the anti-pS366/pS369-PAR4 antibody (7TM0350A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-PAR4 antibody to confirm equal loading of the gel.

Be the first to analyze the PAR4 phosphorylation and let us know. more

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Be the first to analyze the PAR4 phosphorylation and let us know.

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