pS335/pS337-S1P3 (phospho-Sphingosine 1-Phosphate Receptor 3 Antibody)

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Agonist-induced Serine335/Serine337 phosphorylation of the Sphingosine 1-Phosphate Receptor 3

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Order number: 7TM0277A
Content: 100 µl
Host: Rabbit

Serine335/Serine337 (S335/S337) is major phosphorylation site of the Sphingosine... more

Serine335/Serine337 (S335/S337) is major phosphorylation site of the Sphingosine 1-Phosphate Receptor 3 (S1P3). The pS335/pS337-S1P3 antibody detects phosphorylation in response to agonists. S335/S337 phosphorylation is likely to be involved in efficient ligand sequestration by S1P3.

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Alternative Names Edg3, endothelial differentiation G protein-coupled... more

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Alternative Names	Edg3, endothelial differentiation G protein-coupled receptor 3
IUPHAR Target ID	277
UniProt ID	Q99500
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human S1P3 around the phosphorylation site of Ser335/Ser337.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine335/Serine337 phosphorylation of the Sphingosine 1-Phosphate... more

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Figure 1. Agonist-induced Serine335/Serine337 phosphorylation of the Sphingosine 1-Phosphate Receptor 3. Upper panel, HEK293 cells stably expressing the S1P3-Receptor (S1P3) were either not exposed or exposed to 1 µM of S1P3-Receptor agonists S1P for 30 minutes. Cells were lysed and immunoblotted with the anti-pS335/pS337-S1P3 antibody (7TM00277A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-S1P3 antibody (7TM00277N) at a dilution of 1:1000 to confirm equal loading of the gel..

Be the first to analyze the S1P1 phosphorylation using our anti-pS351/pS353-S1P1... more

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Be the first to analyze the S1P1 phosphorylation using our anti-pS351/pS353-S1P1 antibody and let us know.

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