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- Order number: 7TM0166N
- Content: 100 µl
- Host: Rabbit
The non-phospho-SUCNR1 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human SUCNR1. It can be used to detect total SUCNR1 receptors in Western blots independent of phosphorylation. The SUCNR1 antibody can also be used to isolate and enrich SUCNR1 receptors from tissue lysates. It also detects SUCNR1 in cultured cells and tissue sections by immunohistochemistry.
| Alternative Names | succinate receptor 1, GPR91, P2Y purinoceptor 1 |
| IUPHAR Target ID | 166 |
| Uniprot ID | Q9BXA5 |
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide corresponding to distal end of carboxy-terminal tail of human SUCNR1/GPR91. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the SUCNR1 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the SUCNR1 Receptor were lysed and immunoblotted with the phosphorylation-independent anti-SUCNR1 antibody (7TM0166N) at a dilution of 1:1000.
Figure 2. Immunohistochemical varification of SUCNR1 Receptor antibody in Pancreas. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-SUCNR1 (non-phospho-SUCNR1 Receptor) antibody (7TM0166N). Sections were then incubated with anti-SUCNR1 (non-phospho-SUCNR1 Receptor) antibody (7TM0166N) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation SUCNR1 receptors were uniformly detected at the plasma membrane of nearly all cells.
Figure 3. Immunohistochemical identification of SUCNR1 Receptor in Neuroendocrine Cells. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SUCNR1 (SUCNR1 Receptor) antibody (7TM0166N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.