5-HT6 (IHC-grade), 5-HT Receptor 6 Antibody

Figure 1. Immunohistochemical varification of 5-Hydroxytryptamine Receptor 6 antibody in Medial Septum. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-5-HT6 (non-phospho-5-HT6 Receptor) antibody (7TM0011N-IC). Sections were then incubated with anti-5HT6 (non-phospho-5-HT6 Receptor) antibody (7TM0011N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation 5-HT6 receptors were uniformly detected at the plasma membrane of nearly all cells.

Figure 2. Validation of the 5-Hydroxytryptamine Receptor 6 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the 5-Hydroxytryptamine Receptor 6 (5-HT6) were lysed and immunoblotted with the phosphorylation-independent anti-5-HT6 antibody (7TM0011N-IC) at a dilution of 1:1000.