GPR12 (IHC-grade), G Protein-Coupled Receptor 12 Antibody

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Preview: Immunohistochemical identification of GPR12 Receptor in kidney

Preview: Validation of the GPR12 Receptor in transfected HEK293 cells

Immunohistochemical identification of GPR12 Receptor in kidney

Validation of the GPR12 Receptor in transfected HEK293 cells

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Order number: 7TM0086N-IC
Content: 100 µl
Host: Rabbit

The GPR12 antibody is directed against the distal end of the carboxyl-terminal tail of mouse,... more

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The GPR12 antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human GRP12. It can be used to detect total GPR12 receptors in Western blots independent of phosphorylation. The GPR12 antibody can also be used to isolate and enrich GPR12 receptors from brain lysates. It also detects GPR12 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names Gpcr01, Gpcr20, GPCR21 IUPHAR Target ID... more

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Alternative Names	Gpcr01, Gpcr20, GPCR21
IUPHAR Target ID	86
UniProt ID	P47775
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to distal end of carboxy-terminal tail of human GPR12.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of GPR12 Receptor in kidney. ... more

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Figure 1. Immunohistochemical identification of GPR12 Receptor in kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR12 (GPR12 Receptor) antibody (7TM0086N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Validation of the GPR12 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR12 Receptor (GPR12) were lysed and immunoblotted with the phosphorylation-independent anti-GPR12 antibody (7TM0086N-IC) at a dilution of 1:1000.

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