![Validation of the 5-Hydroxytryptamine Receptor 6 in transfected HEK293 cells](https://7tmantibodies.com/media/image/97/6c/99/5HT6-NP_600x600.png)
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- Order number: 7TM0011N
- Content: 100 µl
- Host: Rabbit
The non-phospho-5-HT6 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human 5-Hydroxytryptamine Receptor 6 (5-HT6). It can be used to detect total 5-HT6 receptors in Western blots independent of phosphorylation. The non-phospho-5-HT6 antibody can also be used to isolate and enrich 5-HT6 receptors from cell and tissue lysates.
Alternative Names | 5-HT6, 5-Hydroxytryptamine Receptor 6, Serotonin Receptor 6 |
IUPHAR Target ID | 11 |
UniProt ID | P50406 |
Western Blot (WB) | 1:1000 |
Immunohistochemistry (IHC) | - |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic peptide presents the carboxyl-terminal tail of human 5-HT6. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the 5-Hydroxytryptamine Receptor 6 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the 5-Hydroxytryptamine Receptor 6 (5-HT6) were lysed and immunoblotted with the phosphorylation-independent anti-5-HT6 antibody (7TM0011N) at a dilution of 1:1000.