β2-Adrenoceptor Sample Pack (phospho- and non-phospho-β2-Adrenoceptor Antibodies)

SAMPLE PACK

Preview: Agonist-induced Serine355/Serine356 phosphorylation of the β2-Adrenoceptor

Preview: Agonist-induced Threonine360/Serine364 phosphorylation of the β2-Adrenoceptor

Preview: Agonist-induced Serine261/Serine262 phosphorylation of the β2-Adrenoceptor

Preview: Validation of the β2-Adrenoceptor in transfected HEK293 cells

Agonist-induced Serine355/Serine356 phosphorylation of the β2-Adrenoceptor

Agonist-induced Threonine360/Serine364 phosphorylation of the β2-Adrenoceptor

Agonist-induced Serine261/Serine262 phosphorylation of the β2-Adrenoceptor

Validation of the β2-Adrenoceptor in transfected HEK293 cells

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Order number: 7TM0029-SP
Content: 4 x 20 µl
Host: Rabbit

β2-Adrenoceptor Antibody Sample Pack consisting of three phospho- and one... more

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β2-Adrenoceptor Antibody Sample Pack consisting of three phospho- and one non-phospho-β2-Adrenoceptor Antibodies 4 x 20 µL trial size each. Specifically, this sample pack contains the following antibodies pS355/pS356-β2 (7TM0029A), pT360/pS364-β2 (7TM0029B), pS261/pS262-β2 (7TM0029G) and β2 (non-phos) (7TM0029N).

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Alternative Names β2, B2AR, ADRB2R, β2 Adrenergic... more

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Alternative Names	β2, B2AR, ADRB2R, β2 Adrenergic Receptor, β2-Adrenoceptor
IUPHAR Target ID	29
UniProt ID	P07550
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	Synthetic phospho- and non-phosphopeptides derived from human β2.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine355/Serine356 phosphorylation of the β2-Adrenoceptor.... more

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Figure 1. Agonist-induced Serine355/Serine356 phosphorylation of the β2-Adrenoceptor. Upper panel, HEK293 cells stably expressing the β2-Adrenoceptor (β2) were either not exposed or exposed to 1 µM of specific β2-adrenoceptor agonist Isoprenaline or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pS355/pS356-β2 antibody (7TM0029A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-β2 antibody (7TM0029N) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Agonist-induced Threonine360/Serine364 phosphorylation of the β2-Adrenoceptor. Upper panel, HEK293 cells stably expressing the β2-Adrenoceptor (β2) were either not exposed or exposed to 1 µM of specific β2-adrenoceptor agonist Isoprenaline or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pT360/pS364-β2 antibody (7TM0029B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-β2 antibody (7TM0029N) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 3. Agonist-induced Serine261/Serine262 phosphorylation of the β2-Adrenoceptor. Upper panel, HEK293 cells stably expressing the β2-Adrenoceptor (β2) were either not exposed or exposed to 1 µM of specific β2-adrenoceptor agonist Isoprenaline for 30 minutes. Cells were lysed and immunoblotted with the anti-pS261/pS262-β2 antibody (7TM0029G) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-β2 antibody (7TM0029N) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 4. Validation of the β2-Adrenoceptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the β2-Adrenoceptor (β2) were lysed and immunoblotted with the phosphorylation-independent anti-β2 antibody (7TM0029N-WB) at a dilution of 1:1000.

Benkel T, Zimmermann M, Zeiner J, Bravo S, Merten N, Lim VJY, Matthees ESF, Drube J,... more

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Benkel T, Zimmermann M, Zeiner J, Bravo S, Merten N, Lim VJY, Matthees ESF, Drube J, Miess-Tanneberg E, Malan D, Szpakowska M, Monteleone S, Grimes J, Koszegi Z, Lanoiselée Y, O'Brien S, Pavlaki N, Dobberstein N, Inoue A, Nikolaev V, Calebiro D, Chevigné A, Sasse P, Schulz S, Hoffmann C, Kolb P, Waldhoer M, Simon K, Gomeza J, Kostenis E. How Carvedilol activates β2-adrenoceptors. Nat Commun. 2022 Nov 19;13(1):7109. doi: 10.1038/s41467-022-34765-w. PMID: 36402762; PMCID: PMC9675828.

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