![Agonist-induced Serine335/Threonine336 phosphorylation of the Angiotensin Receptor 1](https://7tmantibodies.com/media/image/50/e9/2b/pS335-pT336-AT1-F1_600x600.png)
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- Order number: 7TM0034B
- Content: 100 µl
- Host: Rabbit
Serine335/Threonine336 (S335/T336) is major phosphorylation site of the Angiotensin Receptor 1 (AT1). The pS335/pT336-AT1 antibody detects phosphorylation in response to agonists. S335/T336 phosphorylation is likely to be involved in efficient ligand sequestration by AT1.
Alternative Names | AT1, AG2S, AT2R1, Angiotensin II type-1 Receptor, type-1B Angiotensin II Receptor |
IUPHAR Target ID | 34 |
UniProt ID | P30556 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human AT1 around the phosphorylation site of Ser335/Thr336. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine335/Threonine336 phosphorylation of the Angiotensin Receptor 1. Upper panel, HEK293 cells stably expressing the Angiotensin receptor 1 (AT1) were either not exposed or exposed to 100 nM Angiotensin II for 30 minutes. Cells were lysed and immunoblotted with the anti-pS335/pT336-AT1 antibody (7TM0034B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-AT1 antibody to confirm equal loading of the gel.
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