pS335/pT336-AT1 (phospho-Angiotensin Receptor 1 Antibody)

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Agonist-induced Serine335/Threonine336 phosphorylation of the Angiotensin Receptor 1

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Order number: 7TM0034B
Content: 100 µl
Host: Rabbit

Serine335/Threonine336 (S335/T336) is major phosphorylation site of the Angiotensin Receptor 1... more

Serine335/Threonine336 (S335/T336) is major phosphorylation site of the Angiotensin Receptor 1 (AT1). The pS335/pT336-AT1 antibody detects phosphorylation in response to agonists. S335/T336 phosphorylation is likely to be involved in efficient ligand sequestration by AT1.

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Alternative Names AT1, AG2S, AT2R1, Angiotensin II type-1 Receptor, type-1B... more

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Alternative Names	AT1, AG2S, AT2R1, Angiotensin II type-1 Receptor, type-1B Angiotensin II Receptor
IUPHAR Target ID	34
UniProt ID	P30556
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human AT1 around the phosphorylation site of Ser335/Thr336.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine335/Threonine336 phosphorylation of the Angiotensin Receptor 1.... more

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Figure 1. Agonist-induced Serine335/Threonine336 phosphorylation of the Angiotensin Receptor 1. Upper panel, HEK293 cells stably expressing the Angiotensin receptor 1 (AT1) were either not exposed or exposed to 100 nM Angiotensin II for 30 minutes. Cells were lysed and immunoblotted with the anti-pS335/pT336-AT1 antibody (7TM0034B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-AT1 antibody to confirm equal loading of the gel.

Be the first to analyze the AT1 phosphorylation using our anti-pS335/pT336-AT1... more

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