AT1 Sample Pack (phospho- and non-phospho-Angiotensin Receptor Antibodies)

SAMPLE PACK

Preview: Agonist-induced Serine331/Threonine332 phosphorylation of the Angiotensin Receptor 1

Preview: Agonist-induced Serine335/Threonine336 phosphorylation of the Angiotensin Receptor 1

Preview: Agonist-induced Serine346/Serine347 phosphorylation of the Angiotensin Receptor 1

Preview: Validation of the Angiotensin Receptor 1 in transfected HEK293 cell

Agonist-induced Serine331/Threonine332 phosphorylation of the Angiotensin Receptor 1

Agonist-induced Serine335/Threonine336 phosphorylation of the Angiotensin Receptor 1

Agonist-induced Serine346/Serine347 phosphorylation of the Angiotensin Receptor 1

Validation of the Angiotensin Receptor 1 in transfected HEK293 cell

$ 300.00 *

Prices plus VAT plus shipping costs

Ready to ship today,
Delivery time appr. 5-8 days

Order number: 7TM0034-SP
Content: 4 x 20 µl
Host: Rabbit

AT1 Sample Pack consisting of all four available phospho- and non-phospho-AT1 Antibodies 4 x 20... more

Close menu

AT1 Sample Pack consisting of all four available phospho- and non-phospho-AT1 Antibodies 4 x 20 µL trial size each. Specifically, this sample pack contains the following antibodies pS331/pT332-AT1 (7TM0034A), pS335/pT336-AT1 (7TM0034B), pS346/pS347-AT1 (7TM0034C) and AT1 (non-phos, C-Term) (7TM0034N).

Available downloads:

Alternative Names AT1, AG2S, AT2R1, Angiotensin II type-1 Receptor, type-1B... more

Close menu

Alternative Names	AT1, AG2S, AT2R1, Angiotensin II type-1 Receptor, type-1B Angiotensin II Receptor
IUPHAR Target ID	34
UniProt ID	P30556
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human AT1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine331/Threonine332 phosphorylation of the Angiotensin Receptor 1.... more

Close menu

Figure 1. Agonist-induced Serine331/Threonine332 phosphorylation of the Angiotensin Receptor 1. Upper panel, HEK293 cells stably expressing the Angiotensin receptor 1 (AT1) were either not exposed or exposed to 100 nM Angiotensin II for 30 minutes. Cells were lysed and immunoblotted with the anti-pS331/pT332-AT1 antibody (7TM0034A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-AT1 antibody to confirm equal loading of the gel.

Figure 2. Agonist-induced Serine335/Threonine336 phosphorylation of the Angiotensin Receptor 1. Upper panel, HEK293 cells stably expressing the Angiotensin receptor 1 (AT1) were either not exposed or exposed to 100 nM Angiotensin II for 30 minutes. Cells were lysed and immunoblotted with the anti-pS335/pT336-AT1 antibody (7TM0034B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-AT1 antibody to confirm equal loading of the gel.

Figure 3. Agonist-induced Serine346/Serine347 phosphorylation of the Angiotensin Receptor 1. Upper panel, HEK293 cells stably expressing the Angiotensin receptor 1 (AT1) were either not exposed or exposed to 100 nM Angiotensin II for 30 minutes. Cells were lysed and immunoblotted with the anti-pS346/pS347-AT1 antibody (7TM0034C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-AT1 antibody to confirm equal loading of the gel.

Figure 4. Validation of the Angiotensin Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Angiotensin Receptor 1 (AT1) were lysed and immunoblotted with the phosphorylation-independent anti-AT1 antibody (7TM0034N) at a dilution of 1:1000.

Close menu