pS351-APLNR (phospho-Apelin Receptor Antibody)

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Agonist-induced Serine351 phosphorylation of the Apelin Receptor

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Order number: 7TM0036C
Content: 100 µl
Host: Rabbit

Serine351 (S351) is major phosphorylation site of the Apelin Receptor (APLNR).... more

Serine351 (S351) is major phosphorylation site of the Apelin Receptor (APLNR). The pS351-APLNR antibody detects phosphorylation in response to agonists. S351 phosphorylation is likely to be involved in efficient ligand sequestration by APLNR.

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Alternative Names APLNR, Angiotensin Receptor-like 1 IUPHAR Target... more

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Alternative Names	APLNR, Angiotensin Receptor-like 1
IUPHAR Target ID	36
UniProt ID	P35414
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human APLNR around the phosphorylation site of Ser351.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine351 phosphorylation of the Apelin Receptor. Upper panel,... more

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Figure 1. Agonist-induced Serine351 phosphorylation of the Apelin Receptor. Upper panel, HEK293 cells stably expressing the Apelin Receptor (APLNR) were either not exposed or exposed to 100 nM Apelin for 30 minutes. Cells were lysed and immunoblotted with the anti-pS351-APLNR antibody (7TM0036C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-APLNR antibody (7TM0036N) to confirm equal loading of the gel.

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