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pT363/pT364-BB3 (phospho-Bombesin Receptor 3 Antibody)

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Agonist-induced Threonine363/Threonine364 phosphorylation of the Bombesin Receptor 3
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  • 7TM0040B
  • 100 µl
  • Rabbit
Threonine363/Threonine360 (T363/T364) is major phosphorylation site of the Bombesin Receptor 3... more

Threonine363/Threonine360 (T363/T364) is major phosphorylation site of the Bombesin Receptor 3 (BB3). The pT363/pT364-BB3 antibody detects phosphorylation in response to agonists. T363/T364 phosphorylation is likely to be involved in efficient ligand sequestration by BB3.

  Alternative Names BB3, Bombesin like Receptor 3 IUPHAR Target ID... more

 

Alternative Names BB3, Bombesin like Receptor 3
IUPHAR Target ID  40
UniProt ID  P32247
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human BB3 around the phosphorylation site of Thr363/Thr364.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threonine363/Threonine364 phosphorylation of the Bombesin Receptor 3.... more

Figure 1. Agonist-induced Threonine363/Threonine364 phosphorylation of the Bombesin Receptor 3. Upper panel, HEK293 cells stably expressing the Bombesin receptor 3 (BB3) were either not exposed or exposed to 100 nM BB3 agonist BA1 for 30 minutes. Cells were lysed and immunoblotted with the anti-pT363/pT364-BB3 antibody (7TM0040B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-BB3 antibody to confirm equal loading of the gel.

 

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