ACKR3 Sample Pack (phospho- and non-phospho-Atypical Chemokine Receptor 3 Antibodies)

SAMPLE PACK

Preview: Agonist-induced Serine355/Serine360 phosphorylation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7

Preview: Agonist-induced Serine350/Threonine352 phosphorylation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7

Preview: Validation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 in transfected HEK293 cells

Agonist-induced Serine355/Serine360 phosphorylation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7

Agonist-induced Serine350/Threonine352 phosphorylation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7

Validation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 in transfected HEK293 cells

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Order number: 7TM0080-SP
Content: 4 x 20 µL
Host: Rabbit

ACKR3 Sample Pack consisting of all four available phospho- and non-phospho-Atypical Chemokine... more

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ACKR3 Sample Pack consisting of all four available phospho- and non-phospho-Atypical Chemokine Receptor 3 Antibodies 4 x 20 µL trial size each. Specifically, this sample pack contains the following antibodies pS350/pT352-ACKR3 (7TM0080A), pS355/pS360-ACKR3 (7TM0080B), ACKR3 (non-phos, C-Term) (7TM0080N) and ACKR3 (non-phos, N-Term) (7TM0080N2).

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Alternative Names ACKR3, CXCR7, Atypical Chemokine Receptor 3, CXC... more

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Alternative Names	ACKR3, CXCR7, Atypical Chemokine Receptor 3, CXC Chemokine Receptor 7
IUPHAR Target ID	80
UniProt ID	P25106 (human) P56485 (mouse)
Western Blot (WB)	1:1000
Species Reactivity	Human, Mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phospho- and non-phosphopeptides derived from human ACKR3.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine355/Serine360 phosphorylation of the Atypical Chemokine... more

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Figure 1. Agonist-induced Serine355/Serine360 phosphorylation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7. Upper panel, HEK293 cells stably expressing the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 (ACKR3/CXCR7) were either not exposed or exposed to 100 nM CXCL12 or 0.1 μM VUF 11207 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS355/pS360-ACKR3/CXCR7 antibody (7TM0080B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-ACKR3/CXCR7 antibody (7TM0080N) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Agonist-induced Serine350/Threonine352 phosphorylation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7. Upper panel, HEK293 cells stably expressing the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 (ACKR3/CXCR7) were either not exposed or exposed to 100 nM CXCL12 or 0.1 μM 0.1 μM VUF 11207 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS350/pT352-ACKR3/CXCR7 antibody (7TM0080A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-ACKR3/CXCR7 antibody (7TM0080N) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 3. Validation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 (ACKR3) were lysed and immunoblotted with the phosphorylation-independent c-terminal anti-ACKR3/CXCR7 antibody (7TM0080N) at a dilution of 1:1000. Note, ACKR3/CXCR7 forms stable dimers and multimers.

Figure 4. Validation of the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Atypical Chemokine Receptor 3/CXC Chemokine Receptor 7 (ACKR3) were lysed and immunoblotted with the phosphorylation-independent n-terminal anti-ACKR3/CXCR7 antibody (7TM0080N2) at a dilution of 1:1000. Note, ACKR3/CXCR7 forms stable dimers and multimers.

Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM,... more

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Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM, Schulz S, Stumm R. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not β-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9. doi: 10.1016/j.celrep.2019.01.049. PubMed PMID: 30726732.

Sánchez-Alcañiz JA, Haege S, Mueller W, Pla R, Mackay F, Schulz S, López-Bendito G, Stumm R, Marín O. Cxcr7 controls neuronal migration by regulating chemokine responsiveness. Neuron. 2011 Jan 13;69(1):77-90. doi: 10.1016/j.neuron.2010.12.006. PubMed PMID: 21220100.

Hoffmann F, Müller W, Schütz D, Penfold ME, Wong YH, Schulz S, Stumm R. Rapid uptake and degradation of CXCL12 depend on CXCR7 carboxyl-terminal serine/threonine residues. J Biol Chem. 2012 Aug 17;287(34):28362-77. doi: 10.1074/jbc.M111.335679. Epub 2012 Jun 26. PubMed PMID: 22736769; PubMed Central PMCID: PMC3436560.