pT353-CCR10 (phospho-Chemokine Receptor 10 Antibody)

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Preview: Agonist-induced Threonine353 phosphorylation of the Chemokine Receptor 10

Preview: Analysis of dose-dependent Chemokine Receptor 10 phosphorylation using two phosphosite-specific antibodies

Agonist-induced Threonine353 phosphorylation of the Chemokine Receptor 10

Analysis of dose-dependent Chemokine Receptor 10 phosphorylation using two phosphosite-specific antibodies

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Order number: 7TM0067A
Content: 100 µl
Host: Rabbit

Threonine353 (T353) is a major phosphorylation site of the CCR10 receptor. The pT353-CCR10... more

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Threonine353 (T353) is a major phosphorylation site of the CCR10 receptor. The pT353-CCR10 antibody detects phosphorylation in response to high- and low-efficacy agonists and after PKC activation. T353 phosphorylation is a key regulator of CCR10 desensitization, β-arrestin recruitment and internalization.

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Alternative Names CCR10, GPR-2, Chemokine Receptor 10 IUPHAR Target... more

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Alternative Names	CCR10, GPR-2, Chemokine Receptor 10
IUPHAR Target ID	67
UniProt ID	P46092
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human CCR10 around the phosphorylation site of Thr353.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine353 phosphorylation of the Chemokine Receptor 10. Upper... more

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Figure 1. Agonist-induced Threonine353 phosphorylation of the Chemokine Receptor 10. Upper panel, HEK293 cells stably expressing the Chemokine Receptor 10 (CCR10) were either not exposed or exposed to 100 nM specific Chemokine receptor 10 agonist CCL27 or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pT353-CCR10 antibody (7TM0067A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CCR10 antibody to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Chemokine Receptor 10 phosphorylation using two phosphosite-specific antibodies. Upper two panels, HEK293 cells stably expressing the Chemokine Receptor 10 (CCR10) were either not exposed or exposed to increasing concentrations of specific Chemokine receptor 10 agonist CCL27 ranging from 3 nM to 100 nM for 30 minutes. Cells were lysed and immunoblotted with the anti-pT353-CCR10 antibody (7TM0067A) or anti-pT355-CCR10 antibody (7TM0067B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CCR10 antibody to confirm equal loading of the gel.

Be the first to decipher the CCR10 phosphorylation barcode and let us know. more

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Be the first to decipher the CCR10 phosphorylation barcode and let us know.

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