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pT353-CCR10 (phospho-Chemokine Receptor 10 Antibody)

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  • 7TM0067A
  • 100 µl
  • Rabbit
Threonine353 (T353) is a major phosphorylation site of the CCR10 receptor. The pT353-CCR10... more

Threonine353 (T353) is a major phosphorylation site of the CCR10 receptor. The pT353-CCR10 antibody detects phosphorylation in response to high- and low-efficacy agonists and after PKC activation. T353 phosphorylation is a key regulator of CCR10 desensitization, β-arrestin recruitment and internalization.

  Alternative Names CCR10, GPR-2, Chemokine Receptor 10 IUPHAR Target... more

 

Alternative Names CCR10, GPR-2, Chemokine Receptor 10
IUPHAR Target ID  67
UniProt ID  P46092
Western Blot (WB)  1:1000
Immunocytochemistry (ICC)  -
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human CCR10 around the phosphorylation site of Thr353.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threonine353 phosphorylation of the Chemokine Receptor 10. Upper... more

Figure 1. Agonist-induced Threonine353 phosphorylation of the Chemokine Receptor 10. Upper panel, HEK293 cells stably expressing the Chemokine Receptor 10 (CCR10) were either not exposed or exposed to 100 nM specific Chemokine receptor 10 agonist CCL27 or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pT353-CCR10 antibody (7TM0067A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CCR10 antibody to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Chemokine Receptor 10 phosphorylation using two phosphosite-specific antibodies. Upper two panels, HEK293 cells stably expressing the Chemokine Receptor 10 (CCR10) were either not exposed or exposed to increasing concentrations of specific Chemokine receptor 10 agonist CCL27 ranging from 3 nM to 100 nM for 30 minutes. Cells were lysed and immunoblotted with the anti-pT353-CCR10 antibody (7TM0067A) or anti-pT355-CCR10 antibody (7TM0067B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CCR10 antibody to confirm equal loading of the gel.


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