Premium Phosphosite-Specific 7TM Antibodies
Novel Tools for Your GPCR Research
Select Your Country of Delivery below

pT340-CCR5 (phospho-Chemokine Receptor 5 Antibody)

NEW
$ 375.00 *

Prices plus VAT plus shipping costs

Ready to ship today,
Delivery time appr. 5-8 days

  • 7TM0062B
  • 100 µl
  • Rabbit
Threonine340 (T340) is a major phosphorylation site of the CCR5 receptor. The pT340-CCR5... more

Threonine340 (T340) is a major phosphorylation site of the CCR5 receptor. The pT340-CCR5 antibody detects phosphorylation in response to high- and low-efficacy agonists and after PKC activation. T340 phosphorylation is a key regulator of CCR5 desensitization, β-arrestin recruitment and internalization.

  Alternative Names CCR5, CKR5, Chemokine Receptor 5, Chemokine (C-C motif)... more

 

Alternative Names CCR5, CKR5, Chemokine Receptor 5, Chemokine (C-C motif) Receptor 5, MIP-1 alpha Receptor
IUPHAR Target ID  62
UniProt ID  P51681
Western Blot (WB)  1:1000
Immunocytochemistry (ICC)  -
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human CCR5 around the phosphorylation site of Thr340.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threonine340 phosphorylation of the Chemokine Receptor 5. Upper... more

Figure 1. Agonist-induced Threonine340 phosphorylation of the Chemokine Receptor 5. Upper panel, HEK293 cells stably expressing the Chemokine Receptor 5 (CCR5) were either not exposed or exposed to 100 nM specific Chemokine receptor 5 agonist CCL5 or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pT340-CCR5 antibody (7TM0062B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CCR5 antibody to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Chemokine Receptor 5 phosphorylation using three phosphosite-specific antibodies. Upper three panels, HEK293 cells stably expressing the Chemokine Receptor 5 (CCR5) were either not exposed or exposed to increasing concentrations of specific Chemokine receptor 5 agonist CCL5 ranging from 3 nM to 100 nM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS336/pS337-CCR5 antibody (7TM0062A) or pT340-CCR5 antibody (7TM0062B) or pS342-CCR5 antibody (7TM0062C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CCR5 antibody to confirm equal loading of the gel.

Isaikina P, Petrovic I, Jakob RP, Sarma P, Ranjan A, Baruah M, Panwalkar V, Maier T, Shukla AK,... more

Isaikina P, Petrovic I, Jakob RP, Sarma P, Ranjan A, Baruah M, Panwalkar V, Maier T, Shukla AK, Grzesiek S. A key GPCR phosphorylation motif discovered in
arrestin2⋅CCR5 phosphopeptide complexes. Mol Cell. 2023 Jun 15;83(12):2108-2121.e7. doi: 10.1016/j.molcel.2023.05.002. Epub 2023 May 26. PMID: 37244255.

Read, write and discuss reviews... more
Customer evaluation for "pT340-CCR5 (phospho-Chemokine Receptor 5 Antibody)"
Write an evaluation
Evaluations will be activated after verification.

The fields marked with * are required.

Recently viewed