pT340-CCR5 (phospho-Chemokine Receptor 5 Antibody)

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Preview: Agonist-induced Threonine340 phosphorylation of the Chemokine Receptor 5

Preview: Analysis of dose-dependent Chemokine Receptor 5 phosphorylation using three phosphosite-specific antibodies

Agonist-induced Threonine340 phosphorylation of the Chemokine Receptor 5

Analysis of dose-dependent Chemokine Receptor 5 phosphorylation using three phosphosite-specific antibodies

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Order number: 7TM0062B
Content: 100 µl
Host: Rabbit

Threonine340 (T340) is a major phosphorylation site of the CCR5 receptor. The pT340-CCR5... more

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Threonine340 (T340) is a major phosphorylation site of the CCR5 receptor. The pT340-CCR5 antibody detects phosphorylation in response to high- and low-efficacy agonists and after PKC activation. T340 phosphorylation is a key regulator of CCR5 desensitization, β-arrestin recruitment and internalization.

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Alternative Names CCR5, CKR5, Chemokine Receptor 5, Chemokine (C-C motif)... more

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Alternative Names	CCR5, CKR5, Chemokine Receptor 5, Chemokine (C-C motif) Receptor 5, MIP-1 alpha Receptor
IUPHAR Target ID	62
UniProt ID	P51681
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human CCR5 around the phosphorylation site of Thr340.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine340 phosphorylation of the Chemokine Receptor 5. Upper... more

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Figure 1. Agonist-induced Threonine340 phosphorylation of the Chemokine Receptor 5. Upper panel, HEK293 cells stably expressing the Chemokine Receptor 5 (CCR5) were either not exposed or exposed to 100 nM specific Chemokine receptor 5 agonist CCL5 or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pT340-CCR5 antibody (7TM0062B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CCR5 antibody to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Chemokine Receptor 5 phosphorylation using three phosphosite-specific antibodies. Upper three panels, HEK293 cells stably expressing the Chemokine Receptor 5 (CCR5) were either not exposed or exposed to increasing concentrations of specific Chemokine receptor 5 agonist CCL5 ranging from 3 nM to 100 nM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS336/pS337-CCR5 antibody (7TM0062A) or pT340-CCR5 antibody (7TM0062B) or pS342-CCR5 antibody (7TM0062C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CCR5 antibody to confirm equal loading of the gel.

Isaikina P, Petrovic I, Jakob RP, Sarma P, Ranjan A, Baruah M, Panwalkar V, Maier T, Shukla AK,... more

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Isaikina P, Petrovic I, Jakob RP, Sarma P, Ranjan A, Baruah M, Panwalkar V, Maier T, Shukla AK, Grzesiek S. A key GPCR phosphorylation motif discovered in
arrestin2⋅CCR5 phosphopeptide complexes. Mol Cell. 2023 Jun 15;83(12):2108-2121.e7. doi: 10.1016/j.molcel.2023.05.002. Epub 2023 May 26. PMID: 37244255.

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