CXCR4 Sample Pack (phospho- and non-phospho-CXCR4 Chemokine Receptor Antibodies)

SAMPLE PACK

Preview: Agonist-induced Serine324/Serine325 phosphorylation of the CXC Chemokine Receptor 4.

Preview: Agonist-induced Serine330 phosphorylation of the CXC Chemokine Receptor 4

Preview: Agonist-induced Serine338/Serine339 phosphorylation of the CXC Chemokine Receptor 4

Preview: Agonist-induced Serine346/Serine347 phosphorylation of the CXC Chemokine Receptor 4.

Preview: Validation of the CXC Chemokine Receptor 4 in transfected HEK293 cells

Agonist-induced Serine324/Serine325 phosphorylation of the CXC Chemokine Receptor 4.

Agonist-induced Serine330 phosphorylation of the CXC Chemokine Receptor 4

Agonist-induced Serine338/Serine339 phosphorylation of the CXC Chemokine Receptor 4

Agonist-induced Serine346/Serine347 phosphorylation of the CXC Chemokine Receptor 4.

Validation of the CXC Chemokine Receptor 4 in transfected HEK293 cells

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Order number: 7TM0071-SP
Content: 5 x 20 µl
Host: Rabbit

CXCR4 Sample Pack consisting of all five available phospho- and non-phospho-CXCR4... more

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CXCR4 Sample Pack consisting of all five available phospho- and non-phospho-CXCR4 Antibodies 5 x 20 µL trial size each. Specifically, this sample pack contains the following antibodies pS324/pS325-CXCR4 (7TM0071A), pS330-CXCR4 (7TM0071C), pS338/pS339-CXCR4 (7TM0071D), pS346/pS347-CXCR4 (7TM0071B) and CXCR4 (non-phospho), CXC Chemokine Receptor 4 Antibody (7TM0071N).

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Alternative Names CXCR4, CXC Chemokin Receptor 4 IUPHAR Target ID... more

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Alternative Names	CXCR4, CXC Chemokin Receptor 4
IUPHAR Target ID	71
UniProt ID	P61073 (human) P70658 (mouse)
Western Blot (WB)	1:1000
Species Reactivity	Human, Mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human CXCR4
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine324/Serine325 phosphorylation of the CXC Chemokine Receptor 4.... more

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Figure 1. Agonist-induced Serine324/Serine325 phosphorylation of the CXC Chemokine Receptor 4. Upper panel, HEK293 cells stably expressing the HA-tagged CXC Chemokine Receptor 4 were either not exposed or exposed to 100 nM CXCL12 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS324/pS325-CXCR4 antibody (7TM0071A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Agonist-induced Serine330 phosphorylation of the CXC Chemokine Receptor 4. HEK293 cells transiently expressing CXCR4 were either not exposed or exposed to 100 nM CXCL12 for 5 minutes. Cells were lysed and immunoblotted with the anti-pS330 antibody (7TM0071C) at a dilution of 1:1000 (upper panel). The same lysates were probed with the non-phospho-CXCR4 antibody (7TM0071N) at a dilution of 1:1000 (lower panel).

Figure 3. Agonist-induced Serine338/Serine339 phosphorylation of the CXC Chemokine Receptor 4. HEK293 cells transiently expressing CXC Chemokine Receptor 4 (CXCR4) were either not exposed or exposed to 100 nM CXCL12 for 5 minutes. Cells were lysed and immunoblotted with the anti-pS338/pS339-CXCR4 antibody (7TM0071D) at a dilution of 1:1000 (upper panel). The same lysates were probed with the non-phospho-CXCR4 antibody (7TM0071N) at a dilution of 1:1000 (lower panel).

Figure 4. Agonist-induced Serine346/Serine347 phosphorylation of the CXC Chemokine Receptor 4. Upper panel, HEK293 cells stably expressing the HA-tagged CXC Chemokine receptor 4 were either not exposed or exposed to 100 nM CXCL12 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS346/pS347-CXCR4 antibody (7TM0071B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 5. Validation of the CXC Chemokine Receptor 4 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the CXC Chemokine Receptor 4 (CXCR4) were lysed and immunoblotted with the phosphorylation-sensitive anti-CXCR4 antibody (7TM0071N-WB) at a dilution of 1:1000.

Otte M, Kliewer A, Schütz D, Reimann C, Schulz S, Stumm R. CXCL14 is no directmodulator of... more

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Otte M, Kliewer A, Schütz D, Reimann C, Schulz S, Stumm R. CXCL14 is no directmodulator of CXCR4. FEBS Lett. 2014 Dec 20;588(24):4769-75. doi: 10.1016/j.febslet.2014.11.009. Epub 2014 Nov 18. PubMed PMID: 25451233.

Nakai A, Fujimoto J, Miyata H, Stumm R, Narazaki M, Schulz S, Baba Y, Kumanogoh A, Suzuki K. The COMMD3/8 complex determines GRK6 specificity for chemoattractant receptors. J Exp Med. 2019 Jul 1;216(7):1630-1647. doi: 10.1084/jem.20181494. Epub 2019 May 14. PubMed PMID: 31088898; PubMed Central PMCID: PMC6605747.

Luo J, Busillo JM, Stumm R, Benovic JL. G Protein-Coupled Receptor Kinase 3 and Protein Kinase C Phosphorylate the Distal C-Terminal Tail of the Chemokine Receptor CXCR4 and Mediate Recruitment of β-Arrestin. Mol Pharmacol. 2017 Jun;91(6):554-566. doi: 10.1124/mol.116.106468. Epub 2017 Mar 22. PubMed PMID: 28331048; PubMed Central PMCID: PMC5438129.

Mueller W, Schütz D, Nagel F, Schulz S, Stumm R. Hierarchical organization of multi-site phosphorylation at the CXCR4 C terminus. PLoS One. 2013 May 29;8(5):e64975. doi: 10.1371/journal.pone.0064975. Print 2013. PubMed PMID: 23734232; PubMed Central PMCID: PMC3666969.