pS412/pS414-CCK1 (phospho-Cholecystokinin Receptor 1 Antibody)

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Order number: 7TM0076B
Content: 100 µl
Host: Rabbit

Serine412/Serine414 (S412/S414) is a major phosphorylation site of the CCK1 receptor.... more

Serine412/Serine414 (S412/S414) is a major phosphorylation site of the CCK1 receptor. The pS412/pS414-CCK1 antibody detects phosphorylation in response to high-efficacy agonists. S412/S414 phosphorylation is a key regulator of CCK1 desensitization, β-arrestin recruitment and internalization.

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Alternative Names CCK1, CCKAR, Cholecystokinin Receptor 1, Cholecystokinin... more

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Alternative Names	CCK1, CCKAR, Cholecystokinin Receptor 1, Cholecystokinin Receptor A
IUPHAR Target ID	76
UniProt ID	P32238
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human CCK1 around the phosphorylation site Ser412/Ser414.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine412/Serine414 phosphorylation of CCK1 Receptor. Upper panel,... more

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Figure 1. Agonist-induced Serine412/Serine414 phosphorylation of CCK1 Receptor. Upper panel, HEK293 cells stably expressing the CCK1-Receptor (CCK1) were either not exposed or exposed to 1 µM of specific CCK-Receptor agonist CCK-8 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS412/pS414-CCK1 antibody (7TM0076B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-CCK1 antibody (7TM0076N) at a dilution of 1:1000 to confirm equal loading of the gel.