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pS437/pT439-CCK2 (phospho-Cholecystokinin Receptor 2 Antibody)

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pS437/pT439-CCK2 (phospho-Cholecystokinin Receptor 2 Antibody)
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  • 7TM0077C
  • 100 µl
  • Rabbit
Serine437/Threonine439 (S437/T439) is a major phosphorylation site of the CCK2 receptor.... more

Serine437/Threonine439 (S437/T439) is a major phosphorylation site of the CCK2 receptor. The pS437/pT439-CCK2 antibody detects phosphorylation in response to high-efficacy agonists. S437/T439 phosphorylation is a key regulator of CCK2 desensitization, β-arrestin recruitment and internalization.

  Alternative Names CCK2, CCKBR, Cholecystokinin Receptor 2, Cholecystokinin... more

 

Alternative Names CCK2, CCKBR, Cholecystokinin Receptor 2, Cholecystokinin Receptor B
IUPHAR Target ID  77
UniProt ID  P32239
Western Blot (WB)  1:1000
Immunocytochemistry (ICC)  -
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human CCK2 around the phosphorylation site Ser437/Thr439.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine437/Threonine439 phosphorylation of CCK2 Receptor. Upper... more

Figure 1. Agonist-induced Serine437/Threonine439 phosphorylation of CCK2 Receptor. Upper panel, HEK293 cells stably expressing the CCK2-Receptor (CCK2) were either not exposed or exposed to 1 µM of specific CCK-Receptor agonist CCK-8 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS437/pT439-CCK2 antibody (7TM0077C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-CCK2 antibody (7TM0077N) at a dilution of 1:1000 to confirm equal loading of the gel.

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