pT427/pS429-CCK2 (phospho-Cholecystokinin Receptor 2 Antibody)

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Order number: 7TM0077A
Content: 100 µl
Host: Rabbit

Threonine427/Serine429 (T427/S429) is a major phosphorylation site of the CCK2... more

Threonine427/Serine429 (T427/S429) is a major phosphorylation site of the CCK2 receptor. The pT427/pS429-CCK2 antibody detects phosphorylation in response to high-efficacy agonists. T427/S429 phosphorylation is a key regulator of CRF1 desensitization, β-arrestin recruitment and internalization. T427/S429 also undergoes constitutive phosphorylation.

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Alternative Names CCK2, CCKBR, Cholecystokinin Receptor 2, Cholecystokinin... more

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Alternative Names	CCK2, CCKBR, Cholecystokinin Receptor 2, Cholecystokinin Receptor B
IUPHAR Target ID	77
UniProt ID	P32239
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human CCK2 around the phosphorylation site Thr427/Ser429.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced and Agonist-independent Threonine427/Serine429 phosphorylation of... more

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Figure 1. Agonist-induced and Agonist-independent Threonine427/Serine429 phosphorylation of CCK2 Receptor. Upper panel, HEK293 cells stably expressing the CCK2-Receptor (CCK2) were either not exposed or exposed to 1 µM of specific CCK-Receptor agonist CCK-8 for 30 minutes. Cells were lysed and immunoblotted with the anti-pT427/pS429-CCK2 antibody (7TM0077A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-CCK2 antibody (7TM0077N) at a dilution of 1:1000 to confirm equal loading of the gel.

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