pT354-D1 (phospho-Dopamine Receptor 1 Antibody)

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pT354-D1 (phospho-Dopamine Receptor 1 Antibody)

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Order number: 7TM0214A
Content: 100 µl
Host: Rabbit

Threonine354 (T354) is a major phosphorylation site of the D1 receptor. The pT354-D1... more

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Threonine354 (T354) is a major phosphorylation site of the D1 receptor. The pT354-D1 antibody detects phosphorylation in response to high-efficacy agonists but not after PKC activation. T354 phosphorylation is a key regulator of D1 desensitization, β-arrestin recruitment and internalization.

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Alternative Names D1, DADR, Dopamine Receptor 1, Dopamine-1A Receptor 1... more

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Alternative Names	D1, DADR, Dopamine Receptor 1, Dopamine-1A Receptor 1
IUPHAR Target ID	214
UniProt ID	P21728
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human D1 around the phosphorylation site of Thr354.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine354 phosphorylation of the Dopamine Receptor 1. Upper... more

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Figure 1. Agonist-induced Threonine354 phosphorylation of the Dopamine Receptor 1. Upper panel, HEK293 cells stably expressing the Dopamine Receptor 1 (D1) were either not exposed or exposed to 100 µM Dopamine or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pT354-D1 antibody (7TM0214A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-D1 antibody to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Dopamine Receptor 1 phosphorylation using two phosphosite-specific antibodies. Upper two panels, HEK293 cells stably expressing the Dopamine Receptor 1 (D1) were either not exposed or exposed to increasing concentrations of Dopamine ranging from 1 to 100 µM for 30 minutes. Cells were lysed and immunoblotted with the anti-pT354-D1 antibody (7TM0214A) or anti-pS372/pS373-D1 antibody (7TM0214B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-D1 antibody to confirm equal loading of the gel.

Be the first to decipher the D1 phosphorylation barcode and let us know. more

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Be the first to decipher the D1 phosphorylation barcode and let us know.

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