D1 [UMB10] (RM-IHC-grade), Dopamine Receptor 1 Antibody, Rabbit Monoclonal

Rabbit Monoclonal

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Preview: Immunohistochemical identification of Dopamine Receptor 1 in striatum

Preview: Validation of the Dopamine Receptor 1 in transfected HEK293 cells

Immunohistochemical identification of Dopamine Receptor 1 in striatum

Validation of the Dopamine Receptor 1 in transfected HEK293 cells

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Order number: 7TM0214N-ICRM
Content: 100 µl
Host: Rabbit

The non-phospho-D1 receptor antibody is directed against the distal part of the... more

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The non-phospho-D1 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human D1. It can be used to detect total D1 receptors in Western blots independent of phosphorylation. The non-phospho-D1 antibody can also be used to isolate and enrich D1 receptors from cell and tissue lysates. It also detects D1 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names D1, DADR, Dopamine Receptor 1, Dopamine-1A Receptor 1... more

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Alternative Names	D1, DADR, Dopamine Receptor 1, Dopamine-1A Receptor 1
IUPHAR Target ID	214
UniProt ID	P21728
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Monoclonal
Immunogen	A synthetic peptide presents the carboxyl-terminal tail of human D1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of Dopamine Receptor 1 in striatum. Sections were... more

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Figure 1. Immunohistochemical identification of Dopamine Receptor 1 in striatum. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D1 (Dopamine Receptor 1) antibody (7TM0214N-ICRM) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 2. Validation of the Dopamine Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Dopamine Receptor 1 (D1) were lysed and immunoblotted with the phosphorylation-independent anti-D1 antibody (7TM0214N-ICRM) at a dilution of 1:1000.

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