pS257/pT262-D3 (phospho-Dopamine Receptor 3 Antibody)

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Agonist-induced Serine257/Threonine262 phosphorylation of the D3 Dopamine Receptor

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Order number: 7TM0216A
Content: 100 µl
Host: Rabbit

Serine257/Threonine262 (S257/T262) is a major phosphorylation site of the D3 dopamine receptor.... more

Serine257/Threonine262 (S257/T262) is a major phosphorylation site of the D3 dopamine receptor. The pS257/pT262-D3 antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. S262/T262 phosphorylation is a key regulator of D3 desensitization, β-arrestin recruitment and internalization.

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Alternative Names D3, D3R, Dopaminergic Receptor D3 IUPHAR Target ID... more

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Alternative Names	D3, D3R, Dopaminergic Receptor D3
IUPHAR Target ID	215
UniProt ID	P35462
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human D3 around the phosphorylation site of Ser257/Thr262
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine257/Threonine262 phosphorylation of the D3 Dopamine Receptor.... more

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Figure 1. Agonist-induced Serine257/Threonine262 phosphorylation of the D3 Dopamine Receptor. Upper panel, HEK293 cells stably expressing the D3 Dopamine Receptor (D3) were either not exposed or exposed to 100 nM Dopamine for 5 minutes. Cells were lysed and immunoblotted with the anti-pS257/pT262-D3 antibody (7TM0216A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-D3 antibody to confirm equal loading of the gel.

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