![Agonist-induced Serine257/Threonine262 phosphorylation of the D3 Dopamine Receptor](https://7tmantibodies.com/media/image/05/ca/79/pS257-pT262-D3Yrtf8hXjW0DNM_600x600.png)
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- Order number: 7TM0216A
- Content: 100 µl
- Host: Rabbit
Serine257/Threonine262 (S257/T262) is a major phosphorylation site of the D3 dopamine receptor. The pS257/pT262-D3 antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. S262/T262 phosphorylation is a key regulator of D3 desensitization, β-arrestin recruitment and internalization.
Alternative Names | D3, D3R, Dopaminergic Receptor D3 |
IUPHAR Target ID | 215 |
UniProt ID | P35462 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human D3 around the phosphorylation site of Ser257/Thr262 |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine257/Threonine262 phosphorylation of the D3 Dopamine Receptor. Upper panel, HEK293 cells stably expressing the D3 Dopamine Receptor (D3) were either not exposed or exposed to 100 nM Dopamine for 5 minutes. Cells were lysed and immunoblotted with the anti-pS257/pT262-D3 antibody (7TM0216A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-D3 antibody to confirm equal loading of the gel.