pT382/pT386-D5 (phospho-Dopamine Receptor 5 Antibody)

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Preview: Agonist-induced Threonine382/Threonine386 phosphorylation of the Dopamine Receptor 5

Preview: Analysis of dose-dependent Threonine382/Threonine386 phosphorylation of the Dopamine Receptor 5

Agonist-induced Threonine382/Threonine386 phosphorylation of the Dopamine Receptor 5

Analysis of dose-dependent Threonine382/Threonine386 phosphorylation of the Dopamine Receptor 5

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Order number: 7TM0218A
Content: 100 µl
Host: Rabbit

Threonine382/Threonine386 (T382/T386) is a major phosphorylation site of the D5 receptor.... more

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Threonine382/Threonine386 (T382/T386) is a major phosphorylation site of the D5 receptor. The pT382/pT386-D5 antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. T382/T386 phosphorylation is a key regulator of D5 desensitization, β-arrestin recruitment and internalization.

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Alternative Names D5, DRD1B, Dopamine Receptor 5, D(1B)Dopamine Receptor... more

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Alternative Names	D5, DRD1B, Dopamine Receptor 5, D(1B)Dopamine Receptor
IUPHAR Target ID	218
UniProt ID	P21918
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human D5 around the phosphorylation site of Thr382/Thr386.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine382/Threonine386 phosphorylation of the Dopamine Receptor 5.... more

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Figure 1. Agonist-induced Threonine382/Threonine386 phosphorylation of the Dopamine Receptor 5. Upper panel, HEK293 cells stably expressing the Dopamine Receptor 5 (D5) were either not exposed or exposed to 100 µM Dopamine or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pT382/pT386-D5 antibody (7TM0218A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-D5 antibody to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Threonine382/Threonine386 phosphorylation of the Dopamine Receptor 5. Upper panel, HEK293 cells stably expressing the Dopamine Receptor 5 (D5) were either not exposed or exposed to increasing concentrations of Dopamine ranging from 1 to 100 µM for 30 minutes. Cells were lysed and immunoblotted with the anti-pT382/pT386-D5 antibody (7TM0218A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-D5 antibody to confirm equal loading of the gel.

Be the first to decipher the D5 phosphorylation barcode and let us know. more

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Be the first to decipher the D5 phosphorylation barcode and let us know.

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