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pT334/pS335-FPR3 (phospho-Formylpeptide Receptor 3 Antibody)

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Agonist-induced Threonine334/Serine335 phosphorylation of FPR3 Receptor
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  • 7TM0224A
  • 100 µl
  • Rabbit
Threonine334/Serine335 (T334/S335) is a major phosphorylation site of the FPR3 receptor.... more

Threonine334/Serine335 (T334/S335) is a major phosphorylation site of the FPR3 receptor. The pT334/pS335-FPR3 antibody detects phosphorylation in response to high-efficacy agonists. T334/S335 phosphorylation is a key regulator of FPR3 desensitization, β-arrestin recruitment and internalization.

  Alternative Names FPRL2, FPRH1, Formyl-peptide Receptor-like 2,... more

 

Alternative Names FPRL2, FPRH1, Formyl-peptide Receptor-like 2, Formyl-peptide Receptor 3
IUPHAR Target ID  224
UniProt ID  P25089
Western Blot (WB)  1:1000
Immunocytochemistry (ICC)  -
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human FPR3 around the phosphorylation site Thr334/Ser335.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threonine334/Serine335 phosphorylation of FPR3 Receptor. Upper... more

Figure 1. Agonist-induced Threonine334/Serine335 phosphorylation of FPR3 Receptor. Upper panel, HEK293 cells stably expressing the FPR3-Receptor (FPR3) were either not exposed or exposed to 1 µM of specific FPR-Receptor agonist WKYMVm for 30 minutes. Cells were lysed and immunoblotted with the anti-pT334/pS335-FPR3 antibody (7TM0224A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-FPR3 antibody (7TM0224N) at a dilution of 1:1000 to confirm equal loading of the gel.

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